LOCUS       sequence-72663-e        1068 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of TALEN proteins with FokI-DDD domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1068)
  AUTHORS   Hu R, Wallace J, Dahlem TJ, Grunwald DJ, O'Connell RM
  TITLE     Targeting human microRNA genes using engineered Tal-effector 
            nucleases (TALENs).
  JOURNAL   PLoS One. 2013 May 7;8(5):e63074. doi: 10.1371/journal.pone.0063074.
            Print 2013.
  PUBMED    23667577
REFERENCE   2  (bases 1 to 1068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-7"; volume: "8(5):e63074. doi"; pages: " 
            10.1371/journal.pone.0063074. Print 2013"
COMMENT     Sequence Label: pCS2TAL3-DDD
FEATURES             Location/Qualifiers
     source          1..1068
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(42..629)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQD
                     YVEENQTRDKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
     protein_bind    815..836
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(851..856,857..874,875..881)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    889..905
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     894..916
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     913..929
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     913..929
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     948..968
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        950..968
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        complement(1016..1034)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1044..1061)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1044..1060)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtctggatct acgtaatacg actcactata gttctagatt aaaagtttat ctcgccgtta
       61 ttaaatttcc ggcgcacttc ctctaaggtt aatgtgccgg ctttaatcat ttctccacca
      121 attaaaagct cttctacact aagaacagct ccattacaat tagtgatatg atttaatcgt
      181 gtaagctgag ctttgtagtt tcctttaaag tgaccactca caaataaaaa cttaaattcc
      241 gttacagaag atggatagac tttccaccat tcattagggt tgatatgttt gtctcgtgtt
      301 tgattttctt cgacatagtc ttgcatttca tctgcttggc caattggcag attataacct
      361 ccgctataag ctttagtatc cacgatcaca ccgtaatcaa taggagatcc gacagtataa
      421 attgctccgt ccggtttcct tgatccaccc aaatgtttac ctctatatcc ataaactttc
      481 ataaaaaatt ccattacctt catttcaaga attctatcct gagtggaatt tctggcaatt
      541 tcaattaatt caatatattc atgaggcaca tatttcaatt tatgacgaag ttcagatttc
      601 ttctcctcca gttcactttt gactagttgg gatccggcaa cgcgatggga cgtgcgttcg
      661 ccaatacggc gattgactct tctgatcaat tccggcgcgt gcggcaatcc ctttttcact
      721 gcatccatgg caggacgtcc gccgaggcag gccaaggcga cgaggtggtc gttggagacg
      781 accggtggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      841 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      901 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
      961 actaaaggga acaaaagctg gaggacctcg agggggggcc cggtacccaa ttcgccctat
     1021 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgt
//
