LOCUS       sequence-72664-e         528 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian tdTomato activation reporter for ST1 with 
            GTCCCCTCCACCCCACAGTG protospacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 528)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-tdTom-ST1
FEATURES             Location/Qualifiers
     source          1..528
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        130..168
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     165..189
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      203..212
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             212..235
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(393..411)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gggcgaattg ggccctctag atgcatgctc gagcggccgc cagtgtgatg gatatctgca
       61 gaattcgccc ttgcgacgta gggataacag ggtaatagtg tcccctccac cccacagtgc
      121 aagaaacgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg tttagtgaac
      181 cgtcagatcg cctggagaat tcgccaccat ggactacaag gatgacgacg ataaaacttc
      241 cggtggcgga ctgggttcca ccgtgagcaa gggcgaggag gtcatcaaag agttcatgcg
      301 cttcaaggtg cgcatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
      361 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggcggccc
      421 cctgcccttc gcctgggaca tcctgtcccc ccagttcatg tacggctcca aggcgtacgt
      481 gaagcacccc gccgacatcc ccgattacaa gaagctgtcc ttccccga
//
