LOCUS       sequence-72669-e         982 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 982)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-ST1n-VP64
FEATURES             Location/Qualifiers
     source          1..982
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..30
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 agcagagctc gtttagtgaa ccgtcagatc gcctggagac gccatccacg ctgttttgac
       61 ctccatagaa gacaccggga ccgatccagc ctccggactc tagaggatcg aacccttgcc
      121 accatgagcg acctggtgct gggcctggcc atcggcatcg gcagcgtggg cgtgggcatc
      181 ctgaacaagg tgaccggcga gatcatccac aagaacagtc gcatcttccc tgctgctcag
      241 gctgagaaca acctggtgcg ccgcaccaac cgccagggtc gccggcttgc tcgccgcaag
      301 aagcaccggc gcgtgcgcct gaaccgcctg ttcgaggaga gcggcctgat caccgacttc
      361 accaagatca gcatcaacct gaacccctac cagctgcgcg tgaagggcct gaccgacgag
      421 ctgagcaacg aggagctgtt catcgccctg aagaacatgg tgaagcaccg cggcatcagc
      481 tacctggacg acgccagcga cgacggcaac agcagcgtgg gcgactacgc ccagatcgtg
      541 aaggagaaca gcaagcagct ggagaccaag acccccggcc agatccagct ggagcgctac
      601 cagacctacg gccagctgcg cggcgacttc accgtggaga aggacggcaa gaagcaccgc
      661 ctgatcaacg tgttccccac cagcgcctac cgcagcgagg ccctgcgcat cctgcagacc
      721 cagcaggagt tcaaccccca gatcaccgac gagttcatca accgctacct ggagatcctg
      781 accggcaagc gcaagtacta ccacggcccc ggcaacgaga agagccgcac cgactacggc
      841 cgctaccgca ccagcggcga gaccctggac aacatcttcg gcatcctgat cggcaagtgc
      901 accttctacc ccgacgagtt ccgcgccgcc aaggccagct acaccgccca ggagttcaac
      961 ctgctgaacg acctgaacaa cc
//
