LOCUS       sequence-7267-ex        9470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9470)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 9470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPT-GFP
FEATURES             Location/Qualifiers
     source          1..9470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2033..2150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2206..2417
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      2470..2479
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(2476..2478,2479..2481,2482..3192)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(2476..2478,2479..2481,2482..3192)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2521..2542)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2782..2801)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3129..3150
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(3270..3286)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3271..3287)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3300..3888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3353..3373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3771..3782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(4032..4065)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             4085..4265
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(4290..4308)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4291..4308)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4598..4617)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4831..4850)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4950..4972
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5010..5028)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5096..5200
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5201..5269,5270..6061)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5419..5438)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6232..6820
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6721..6740
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6974..6991
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(7061..7081)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7066..7395
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7246..7381
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          8529..8594
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8724..8744
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    9169..9303
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9206..9225)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9260..9279
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga
     2161 gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat
     2221 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2281 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2341 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2401 ttgccgccag aacacaggtg tcgtgacgcg gatccaggcc taagcttacg cgtcctagcg
     2461 ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     2521 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     2581 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2641 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2701 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2761 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     2821 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2881 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     2941 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     3001 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     3061 ctgcccgaca accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag
     3121 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     3181 gagctgtaca agtccggact cagatctcga ctagctagta gctagctagc tagtcgagct
     3241 caacttcgaa ttcgatatca agcttatcgc gataccgtcg acctcgaggg aattccgata
     3301 atcaacctct ggattacaaa atttgtgaaa gattgactgg tattcttaac tatgttgctc
     3361 cttttacgct atgtggatac gctgctttaa tgcctttgta tcatgctatt gcttcccgta
     3421 tggctttcat tttctcctcc ttgtataaat cctggttgct gtctctttat gaggagttgt
     3481 ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt tgctgacgca acccccactg
     3541 gttggggcat tgccaccacc tgtcagctcc tttccgggac tttcgctttc cccctcccta
     3601 ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt
     3661 tgggcactga caattccgtg gtgttgtcgg ggaagctgac gtcctttcca tggctgctcg
     3721 cctgtgttgc cacctggatt ctgcgcggga cgtccttctg ctacgtccct tcggccctca
     3781 atccagcgga ccttccttcc cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc
     3841 gccttcgccc tcagacgagt cggatctccc tttgggccgc ctccccgcat cgggaattcg
     3901 agctcggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     3961 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga tgggatcaat
     4021 tcaccatggg aataacttcg tatagcatac attatacgaa gttatgctgc tttttgcttg
     4081 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     4141 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     4201 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     4261 tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg
     4321 atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta caagcctaat
     4381 tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga
     4441 gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca cccggaaatg
     4501 gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg acgcatcgtg
     4561 gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat
     4621 ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg
     4681 gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg
     4741 gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat
     4801 aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg catagttaag
     4861 ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc
     4921 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc
     4981 gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     5041 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     5101 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     5161 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     5221 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     5281 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     5341 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     5401 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     5461 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     5521 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     5581 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     5641 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     5701 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     5761 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     5821 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     5881 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     5941 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6001 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     6061 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     6121 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     6181 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     6241 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     6301 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     6361 gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     6421 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     6481 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     6541 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     6601 actgagatac ctacagcgtg agcattgaga aagcgccacg cttcccgaag ggagaaaggc
     6661 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     6721 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     6781 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     6841 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     6901 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     6961 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7021 gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg tcagttaggg
     7081 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     7141 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     7201 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     7261 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     7321 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     7381 ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt tgagaatacc
     7441 actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat gtgaaatact
     7501 ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct cgaacacttt
     7561 ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca tcgtcacctg
     7621 ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg
     7681 gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa
     7741 ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga caaccagcgc
     7801 gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt tattgatgac
     7861 ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt
     7921 gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt tgttgatatc
     7981 ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt cccgccaatc
     8041 tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt taacttcagg
     8101 cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac aggcaaacct
     8161 gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc tagtccgccg
     8221 ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa ccatccctca
     8281 ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat
     8341 cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg atgatttata
     8401 cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc cggatctttg
     8461 tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca gagatttaaa
     8521 gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg attctaattg
     8581 tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg tggaatgcct
     8641 ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat gaggctactg
     8701 ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac cccaaggact
     8761 ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga actcttgctt
     8821 gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa attatggaaa
     8881 aatattctgt aacctttata agtaggcata acagttataa tcataacata ctgttttttc
     8941 ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct
     9001 ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt gccttgacta
     9061 gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     9121 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     9181 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     9241 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     9301 atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc ataccctatt
     9361 accactgcca attacctagt ggtttcattt actctaaacc tgtgattcct ctgaattatt
     9421 ttcattttaa agaaattgta tttgttaaat atgtactaca aacttagtag
//
