LOCUS       sequence-72670-e        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Neomycin/Gancyclovir selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ22
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
       61 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctct agagaagtcc atactaacca
      121 ggacccactt gacgccacca tggcctcgta ccccggccat caacacgcgt ctgcgttcga
      181 ccaggctgcg cgttctcgcg gccatagcaa ccgacgtacg gcgttgcgcc ctcgccggca
      241 gcaagaagcc acggaagtcc gcccggagca gaaaatgccc acgctactgc gggtttatat
      301 agacggtccc cacgggatgg ggaaaaccac caccacgcaa ctgctggtgg ccctgggttc
      361 gcgcgacgat atcgtctacg tacccgagcc gatgacttac tggcgggtgc tgggggcttc
      421 cgagacaatc gcgaacatct acaccacaca acaccgcctc gaccagggtg agatatcggc
      481 cggggacgcg gcggtggtaa tgacaagcgc ccagataaca atgggcatgc cttatgccgt
      541 gaccgacgcc gttctggctc ctcatatcgg gggggaggct gggagctcac atgccccgcc
      601 cccggccctc accctcctcc tcgaccgcca tcccatcgcc gtcatgctgt gctacccggc
      661 cgcgcggtac cttatgggca gcatgacccc ccaggccgtg ctggcgttcg tggccctcat
      721 cccgccgacc ttgcccggca ccaacatcgt gcttggggcc cttccggagg acagacacat
      781 cgaccgcctg gccaaacgcc agcgccccgg cgagcggctg gacctggcta tgctggctgc
      841 gattcgccgc gtttacgggc tacttgccaa tacggtgcgg tatctgcagt gcggcgggtc
      901 gtggcgggag gactggggac agctttcggg gacggccgtg ccgccccagg gtgccgagcc
      961 ccagagcaac gcgggcccac gaccccatat cggggacacg ttatttaccc tgtttcgggc
     1021 ccccgagttg ctggccccca acggcgacct gtataacgtg tttgcctggg cct
//
