LOCUS       sequence-72672-e        1010 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian ST1-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 1010)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-ST1n-VP64
FEATURES             Location/Qualifiers
     source          1..1010
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(44..193)
                     /codon_start=1
                     /product="tetrameric repeat of the minimal activation 
                     domain of herpes simplex virus VP16 (Beerli et al., 1998)"
                     /label=VP64
                     /translation="DALDDFDLDMLGSDALDDFDLDMLGSDALDDFDLDMLGSDALDDF
                     DLDML"
     CDS             complement(218..238)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 attactaacc ggtagggatc gaaccctttc atctagagtt aatcagcatg tccaggtcga
       61 aatcatcaag ggcgtcactg ccgagcatgt cgaggtcaaa gtcatcaagg gcatccgaac
      121 caagcatgtc aaggtcaaaa tcatcgaggg cgtcacttcc cagcatatcc agatcaaaat
      181 cgtccaatgc gtcagcccgt ccggaaccgc tggcctccac cttcctcttc ttcttggggt
      241 cagccctgct gaagtccagc ttgggcttgt cgccctcgtt cttgatgatg tgctggttgc
      301 ccagcacgtc ggtgcgcacc ttgtagatgc tgatgttgct cttgcccagg cccttcttgc
      361 actggccgct gttggccacg ttgcccagca ccttgatcag ggcctcgccg ccctcgaact
      421 tctgcttgtc gtagggcttc agctccacgt agtgcttctg cttgggcatg gtgcggctca
      481 ggaagcggaa cagctgttgc tccttggtct cggtgtcctt caccagcaga aggtcgttct
      541 tgtacagggt gaacttgaac tcgctgtcgc tgtccacgcc ctccttcttc ttgatgtcgt
      601 tgtacttctc ctggctgatc ttgtaggtgc cggtgccctt atcaaactgc aggtcggcgt
      661 acttcaggcc caggatctcg tacttgccgg tggtcttgtt gaagtacacg tcggcgcgcc
      721 aggggctcac gctctgcagc accaccttgt tgttgctgtc cttgggggtg atgtcgatgt
      781 ggttgcccag cttgctgtcg tagtacttca ggctcttgat ctcggggccg ttgcccttct
      841 tgctgtactt gcggatgtag ccgtgctcct ccttgtactt caggaagggg ttgcagggca
      901 cctccttgcc tttatcgttg atctgcttgt tggggtagtt ctccaggatg ggctcgatca
      961 ccttctcgaa ggtctggggg tcgtggcggt acatcaggaa cttgctcttg
//
