LOCUS       sequence-72674-e        1077 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Neomycin/Gancyclovir selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1077)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1077)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ22
FEATURES             Location/Qualifiers
     source          1..1077
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    288..321
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 agcggccgga gaacctgcgt gcaatccatc caagcttggc cggatcacct cccagaactt
       61 tgagccgatc tggttaccgc attggccagc ctgaacgcag acgatttcgc gcatattcga
      121 gatctgtaat gtcagtgcga gcgatcaaga atgtaacaca aaggaatacc aagtttgtga
      181 attttatttt ttggtgtaat tgaagtcttc aagttgaaaa aaatggggaa aaggaaaaaa
      241 aactcgaagg cgcgtgcagc ggccggccga cgagaacgtg taccggtata acttcgtata
      301 atgtatgcta tacgaagtta taagcttcgt tgcagttgag tttctgtttt cgggcttgtt
      361 ggtatacttg tttgattaat gtggctgata gctaagctat cgcaaatatt tgtacaagac
      421 atgagggggc aaaaaaataa gccaagtgta tttgttaggc ttccgtgcct gttagagaaa
      481 tatgtaagga aacgcgtgcg ctacacagct tgagcaccct cgagcatctt cattacctac
      541 tgctaatgac gaacgggaaa tgccccgtcc gcgtctagta ttgctcctcc tcgtcgaact
      601 cgccctcctc ctcaatcgtg gcatcctggt actgctggta ctcagacacg agatcgttca
      661 tgttggactc tgcctccgtg aattccatct cgtccatacc ctcgccagtg taccagtgca
      721 agaacgcctt gcgacggaac atgagggtga actgctctcc cacacggcgg aacatctcct
      781 ggatgcaggt gttgttgcca atgaaggtga cagccatctt gagtcccttg ggtgggatat
      841 cgcaaacaga ggacttgatg ttgttcggga tctccactca atgaagtagg acgagttctt
      901 gttctgcacg ttcagcatct gctcatcaac ctccttcgtc gacatgcggc cgcggaagag
      961 tgcagacgct gtcaggtagc ggccgtgacg aggatctgca gcttgcatca tgtttttcgc
     1021 atcgaacatc tgctgcgtta gctcgggcac ggagagaccg cggtactgct gcgagcc
//
