LOCUS       sequence-72678-e         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-intergenic region of TUB-HYG (modified from PMID 16269191).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pDS68
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(523..544)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     872..891
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
       61 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcactagt tctagatcaa
      121 gcttaagcta gcaattcgtt tgaactattt tctttgatga aagggttact attctttctc
      181 ctctagtagg gctggtcgag aaaacaagat tgaaagaaaa ggcggaaagg gagagggaga
      241 aagaggtgtg acacgacagg aagaagcgaa taaggggaag gaaggcatac acgcaaccac
      301 agacgtgaca gggtgcctcc tacacaaagg gtgagcaaaa aaacaagaaa aacataggtc
      361 ccaagcgaat taaaaacatt tgagacagaa aacaaattca caaaagaaag aaagaaagaa
      421 cataaatgaa aacctacaca tggtgcgacg ttgtcacact ttgtcgactt agcggtaacg
      481 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      541 catgtgatcg cgcttctcgt tggggtcttt gctcagggcg gactgggtgc tcaggtagtg
      601 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
      661 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
      721 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
      781 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
      841 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
      901 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
      961 catgtggtcg gggtagcggc tgaagcactg cacgc
//
