LOCUS       sequence-72679-e        1069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GFP-intergenic region of TUB-HYG (modified from PMID 16269191).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pDS68
FEATURES             Location/Qualifiers
     source          1..1069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      177..186
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      181..190
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(220..239)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(221..239)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(246..263)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(246..262)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(255..277)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      403..858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(490..509)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     700..721
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        936..1040
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gatgcgacgc aatcgtccga tccggagccg ggactgtcgg gcgtacacaa atcgcccgca
       61 gaagcgcggc cgtctggacc gatggctgtg tagaagtact cgccgatagt ggaaaccgac
      121 gccccagcac tcgtccgagg gcaaaggaat cttctagaaa aaatcgaatt cccgcggccg
      181 ccatggcggc cgggagcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac
      241 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      301 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      361 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
      421 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      481 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      541 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
      601 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
      661 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
      721 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
      781 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
      841 tattaacgct tacaatttcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
      901 acaccgcatc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
      961 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     1021 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccct
//
