LOCUS       sequence-72684-e         900 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial NM crRNA expression: targets NM to protospacer B 
            (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 276 to 310)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 900)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: PM-NM!TB
FEATURES             Location/Qualifiers
     source          1..900
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        276..310
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      complement(485..512)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(604..690)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     843..860
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
       61 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
      121 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
      181 agcggaaccg ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag
      241 cccggcgaca atgaaaacgt tagtcatggc gcgccttgac ggctagctca gtcctaggta
      301 cagtgctagc ttaatgctcg cacatagcag aactttaaaa gtattcgcca tgttgtagct
      361 ccctttctca tttcgcagtg ctacaatccg ccgctatggt cccacgtaga gcatacggaa
      421 aaaaaagtca aaagcctccg accggaggtc ggccttactt gctagcagag tttgtagaaa
      481 cgcaaaaagg ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg
      541 cgggcgtcct gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg
      601 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
      661 gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg
      721 gagaccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag
      781 gtgggaccac cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt
      841 ctgatttaat ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ccccgtagaa
//
