LOCUS       sequence-72687-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial SP crRNA expression: targets SP to protospacer B 
            (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 275 to 309)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: PM-SP!TB
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        275..309
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     repeat_region   351..386
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     terminator      complement(484..511)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(603..689)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
       61 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
      121 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
      181 gcggaaccgc tctagatttc agtgcaattt atctcttcaa atgtagcacc tgaagtcagc
      241 ccggcgacaa tgaaaacgtt agtcatggcg cgccttgacg gctagctcag tcctaggtac
      301 agtgctagct taatgctcgc acatagcaga actttaaaag tattcgccat gttttagagc
      361 tatgctgttt tgaatggtcc caaaacccgc cgctatggtc ccacgtagag catacggaaa
      421 aaaaagtcaa aagcctccga ccggaggtcg gccttacttg ctagcagagt ttgtagaaac
      481 gcaaaaaggc catccgtcag gatggccttc tgcttaattt gatgcctggc agtttatggc
      541 gggcgtcctg cccgccaccc tccgggccgt tgcttcgcaa cgttcaaatc cgctcccggc
      601 ggatttgtcc tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag
      661 tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg
      721 agaccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg
      781 tgggaccacc gcgctactgc cgccaggcaa attctgtttt atcagaccgc ttctgcgttc
      841 tgatttaatc tgtatc
//
