LOCUS       sequence-72689-e        5269 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA right border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5269)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 5269)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGY001
FEATURES             Location/Qualifiers
     source          1..5269
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    534..556
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             2117..2776
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2183..2202)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2795..3100
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2979..2998
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(3130..3152)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(3346..3365)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    3469..3493
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     rep_origin      complement(3584..4172)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3664..3683)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(4417..4950)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQARS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     rep_origin      join(5241..5269,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaga tcttggcagg
      541 atatattgtg gtgtaacgtt ctcgaactag gctcggacga agtaagcggc cgcaacctta
      601 attaaacacg gcgcgccaac agcgatcgca ccaggtacca ccttgagacc gaattcgctt
      661 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
      721 tactgatatg tatacccgaa gtatgttgga tttgccccta tatttccaga catctgttat
      781 cacttaaccc attacaagcc cgctgccgca gatattcccg tggcgagcga taacccagcg
      841 cactatgcgg atgccattcg ttataatgct cgaacgcctc tgcaaggttc tttgctgccg
      901 ttaacccgtc tggtttgggc atgatactga tgtagtcacg ctttatcgtt ttcacgaagc
      961 tctctgctat tccgttactc tccggactcc gcaccgccgt gttcttcggt tcaagtccca
     1021 acatccgggc gaactggcgt gtttcattag cccggtagca tgaaccatta tccgtcagcc
     1081 actccactgg agacgacgga agatcgttgc cgaagcggcg ttccaccgct cccagcatga
     1141 cgtcctgtac tgtttcactg ttgaagccgc cggtagtgac cgcccagtgc agtgcctcac
     1201 gatcacagca gtccagcgcg aacgtgacac gcagtctctc tccgttatca cagcagaact
     1261 cgaacccgtc agagcaccat cgctgattgc tttctttcac ggccactctg cctgtatgtg
     1321 cccgtttcga tggcggtaca gcaggttttc gctcaagcaa cagcgcattc tggcgcatga
     1381 tccggtaaac acgtttggca ttgatcgcag gcataccatc aagttctgcc tgtctgcgaa
     1441 gcagcgccca tacccgacga taaccatacg ttggcagctc tccgataaca tggtgtatac
     1501 ggagaagcac atccgtatca tcagtgtgac gactgcggcg gccatccatc cagtcatcgg
     1561 ttcgtctgag aatgacgtgc aactgcgcac gcgacacccg gagacaacgg ctgactaagc
     1621 ttactcccca tccccgggca ataagggcgc gtgcgctatc cacttttttg cccgtccata
     1681 ttcaacggct tctttgagga gttcattttc catcgttttc ttgccgagca ggcgctggag
     1741 ttctttaatc tgcttcatgg cggcagcaag ttcagaggca ggaacaacct gttctccggc
     1801 ggcgacagca gtaagacttc cttcctggta ttgcttacgc cagagaaata actggctggc
     1861 tgctacacca tgttgccggg caacgaggga gaccgtcatc cccggttcaa agctctgctg
     1921 aacaattgcg atcttttcct gtgtggtacg ccgtctgcgt ttctccggcc ctaagacatc
     1981 aatcatctgt tctccaatga ctagtctaaa aactagtatt aagactatca cttatttaag
     2041 tgatattggt tgtctggaga ttcagggggc cagtctatat gtcaaaaaga ggtgtgctaa
     2101 gcttaggagg tcagctatgg agaaaaaaat cactggatat accaccgttg atatatccca
     2161 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     2221 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     2281 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg agttccgtat
     2341 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     2401 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     2461 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     2521 taaagggttt attgagaata tgtttttcgt ctcagccaat ccctgggtga gtttcaccag
     2581 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     2641 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     2701 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     2761 gcagggcggg gcgtaagagc tcaggaggac agctatgcag tttaaggttt acacctataa
     2821 aagagagagc cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg
     2881 gcgacggatg gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga
     2941 actttaccca gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc
     3001 cagtgtgcca gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga
     3061 catcaaaaac gccattaacc tgatgttctg gggaatataa actagtagga ggtaatcaat
     3121 gctggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg
     3181 ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg
     3241 cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct
     3301 tacgcatctg tgcggtattt cacaccgcat atggtgcact ctcagtacaa tctgctctga
     3361 tgccgcatga tcatctggcg gacctcttcg gaggtccgct tttttttctc gagggtctca
     3421 gtattctaga ccaagcatgc acagctggcg ttctcagcca gatccccttg acaggatata
     3481 ttggcgggta aactaagtcg ctgtatgtgt ttgtttgaga tctcatgtga gcaaaaggcc
     3541 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     3601 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     3661 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     3721 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     3781 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     3841 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     3901 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     3961 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     4021 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga agaagagttg
     4081 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     4141 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     4201 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     4261 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     4321 atgtgtaaca ttggtctagt gattagggcc gcggcgttgt gacaattttt ccgaacaact
     4381 ccgcggccgc gaagcgatct cggcttgaac gaattgttag gtggcggtac ttgggtcgat
     4441 atcaaagtgc atcacttctt cccgtatgcc caactttgta tagagagcca ctgcgggatc
     4501 gtcaccgtaa tctgcttgca cgtagatcac ataagcacca agcgcgttgg cctcatgctt
     4561 gaggagattg atgagcgcgg tggcaatgcc ttgcctccgg tgctcgccgg agactgcgag
     4621 atcatagata tagatctcac tacgcgcctg ctcaaacttt ggcagaacgt aagccgcgag
     4681 agcgccaaca accgcttctt ggtcgaaggc agcaagcgcg atgaatgtct tactacggag
     4741 caagttcccg aggtaatcgg agtccggctg atgttgggag taggtggcta cgtctccgaa
     4801 ctcacgaccg aaaagatcaa gagcagccct catggatttg acttggtcag ggccgagcct
     4861 acatgtgcga atgatgccca tacttgagcc acctaacttt gttttagggc gactgccctg
     4921 ctgcgtaaca tcgttgctgc tgcgtaacat aacacccctt gtattactgt ttatgtaagc
     4981 agacagtttt attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt
     5041 ttgagacaca acgtggcttt gttgaataaa tcgaactttt gctgagttga aggatcagat
     5101 cacgcatctt cccgacaacg cagaccgttc cgtggcaaag caaaagttca aaatcaccaa
     5161 ctggtccacc tacaacaaag ctctcatcaa ccgtggctcc ctcactttct ggctggatga
     5221 tggggcgatt caggcgatcc ccatccaaca gcccgccgtc gagcgggct
//
