LOCUS       sequence-72691-e         830 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial ST1 repression YFP reporter: protospacer B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: SK-YFP-ST1-B
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     198..216
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(401..429)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccgacgt ctaagaaacc attattatca
      181 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagggga
      241 tccgataaat atctaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt
      301 gcatgtacta gaattctttc tttaagaagg agatatacat atgaattctt catggcgaat
      361 acttttaaag tctcgtaaag gagaagaact tttcactgga gttgtcccaa ttcttgttga
      421 attagatggt gatgttaatg ggcacaaatt ttctgtcagt ggagagggtg aaggtgatgc
      481 aacatacgga aaacttaccc ttaaatttat ttgcactact ggaaaactac ctgttccatg
      541 gccaacactt gtcactactt tcggttatgg tctaaaatgc tttgctagat acccagatca
      601 tatgaaacgg catgactttt tcaagagtgc catgcccgaa ggttatgtac aggaaagaac
      661 tatatttttc aaagatgacg ggaactacaa gacacgtgct gaagtcaagt ttgaaggtga
      721 tacccttgtt aatagaatcg agttaaaagg tattgatttt aaagaagatg gaaacattct
      781 tggacacaaa ttggaataca actataactc acacaatgta tacatcatgg
//
