LOCUS       sequence-72692-e         855 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial SP and NM repression YFP reporter: protospacer B.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: SK-YFP-SPNM-B
FEATURES             Location/Qualifiers
     source          1..855
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     198..216
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(403..431)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccgacgt ctaagaaacc attattatca
      181 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagggga
      241 tccgataaat atctaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt
      301 gcatgtacta gaattcttta actttaagaa ggagatatac atatgaatcc cctatggcga
      361 atacttttaa agtctcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      421 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      481 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      541 tggccaacac ttgtcactac tttcggttat ggtctaaaat gctttgctag atacccagat
      601 catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
      661 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
      721 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
      781 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
      841 caaaagaatg gaatc
//
