LOCUS       sequence-72694-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial TD crRNA expression: targets TD to protospacer B 
            (ACTTTAAAAGTATTCGCCAT), p15A/chloramphenicol.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 266 to 300)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: PM-TD!TB
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        266..300
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     terminator      complement(475..502)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(594..680)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     833..850
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
       61 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
      121 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcggaaccg
      181 ctctagattt cagtgcaatt tatctcttca aatgtagcac ctgaagtcag cccggcgaca
      241 atgaaaacgt tagtcatggc gcgccttgac ggctagctca gtcctaggta cagtgctagc
      301 ttaatgctcg cacatagcag aactttaaaa gtattcgcca tgtttgagag ttgtgtaatt
      361 taagatggat ctcaaacccg ccgctatggt cccacgtaga gcatacggaa aaaaaagtca
      421 aaagcctccg accggaggtc ggccttactt gctagcagag tttgtagaaa cgcaaaaagg
      481 ccatccgtca ggatggcctt ctgcttaatt tgatgcctgg cagtttatgg cgggcgtcct
      541 gcccgccacc ctccgggccg ttgcttcgca acgttcaaat ccgctcccgg cggatttgtc
      601 ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca gtctttcgac
      661 tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg gagaccccac
      721 actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag gtgggaccac
      781 cgcgctactg ccgccaggca aattctgttt tatcagaccg cttctgcgtt ctgatttaat
      841 ctgtatcagg ctgaaaatct tctctcatcc gccaaaacag ccccgtagaa
//
