LOCUS       sequence-72696-e        1004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets TbMCM-BP for Cre-lox mediated knockout in T. brucei.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1004)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pSY45
FEATURES             Location/Qualifiers
     source          1..1004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    287..320
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
       61 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcatttac caacagtaag
      121 atctgtaatg tcagtgcgag cgatcaagaa tgtaacacaa aggaatacca agtttgtgaa
      181 ttttattttt tggtgtaatt gaagtcttca agttgaaaaa aatggggaaa aggaaaaaaa
      241 actcgaaggc gcgtgcagcg gccggccgac gagaacgtgt accggtataa cttcgtataa
      301 tgtatgctat acgaagttat aagcttctgc acttcttcag aaagtaaatg aaaaaagaaa
      361 aagagcttcg ccaaatcctc tcatctttcc agtagtagaa ctgtgaaaaa aagggggggc
      421 acgcaagtat actactattt ccacaaaaaa aaaagaagac agttgcaacg tacataaaat
      481 cacaggtagc aaagtgttcc ccacatgaaa agtgcgcatg tatcaaatgt agagggtagg
      541 gaagcaaaaa tacaaaaata aataaataaa ggtggcactg atgatgtcta actacatttg
      601 aggtggagat tgccacctga atacaacaac aacagacacc actaatcaag ccatatatca
      661 agtggttctt gcacatgcgg ccctttgttc aagtatccaa tgacatggga gacaaacttc
      721 tcgtctcccc gctggaacgg ttgatagcgc ttgagtagtg cccgtaaaag tttatcaccc
      781 tctaaatatg cacccttcca tagacatatt ttatctacca cctcttgatc cctttgcttc
      841 aagacatacg ccattgtggc atcatcaact gacttcagca gtgctaaact ctcgcagttc
      901 tccctctgat tttgttctat ttgcagctgg tagttgtcaa atagcttcat cagtgccata
      961 cgcttctccc gtcgacagct gcattaatga atcggccaac gcgc
//
