LOCUS       sequence-72697-e         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the DHFR tag 
            (c-terminal histag) by homologous recombination and selection for 
            hygromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             35..511
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             512..529
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatgatc agtctgattg cggcgttagc
       61 ggtagatcgc gttatcggca tggaaaacgc catgccgtgg aacctgcctg ccgatctcgc
      121 ctggtttaaa cgcaacacct taaataaacc cgtgattatg ggccgccata cctgggaatc
      181 aatcggtcgt ccgttgccag gacgcaaaaa tattatcctc agcagtcaac cgggtacgga
      241 cgatcgcgta acgtgggtga agtcggtgga tgaagccatc gcggcgtgtg gtgacgtacc
      301 agaaatcatg gtgattggcg gcggtcgcgt ttatgaacag ttcttgccaa aagcgcaaaa
      361 actgtatctg acgcatatcg acgcagaagt ggaaggcgac acccatttcc cggattacga
      421 gccggatgac tgggaatcgg tattcagcga attccacgat gctgatgcgc agaactctca
      481 cagctattgc tttgagattc tggagcggcg gcatcatcat catcatcatt aactcgagca
      541 gctgaagctt gcatgcctgc aggtcgactc tagaggatcc ccgggttaat taaggcgcgc
      601 cagatctgtt tagcttgcct cgtccccgcc gggtcacccg gccagcgaca tggaggccca
      661 gaataccctc cttgacagtc ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc
      721 gtacatttag cccatacatc cccatgtata atcatttgca tccatacatt ttgatggccg
      781 cacggcgcga agcaaaaatt acggctcc
//
