LOCUS       sequence-72698-e        1038 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the DHFR tag 
            (c-terminal histag) by homologous recombination and selection for 
            hygromycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1038)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 1038)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH1
FEATURES             Location/Qualifiers
     source          1..1038
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(84..281)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ttgttagcag ccggatctga tatcatcgat gaattcgagc tcgttttcga cactggatgg
       61 cggcgttagt atcgaatcga cagcagtata gcgaccagca ttcacatacg attgacgcat
      121 gatattactt tctgcgcact taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa
      181 atataaatca cgctaacatt tgattaaaat agaacaacta caatataaaa aaactataca
      241 aatgacaagt tcttgaaaac aagaatcttt ttattgtcag tactgattat tcctttgccc
      301 tcggacgagt gctggggcgt cggtttccac tatcggcgag tacttctaca cagccatcgg
      361 tccagacggc cgcgcttctg cgggcgattt gtgtacgccc gacagtcccg gctccggatc
      421 ggacgattgc gtcgcatcga ccctgcgccc aagctgcatc atcgaaattg ccgtcaacca
      481 agctctgata gagttggtca agaccaatgc ggagcatata cgcccggagc cgcggcgatc
      541 ctgcaagctc cggatgcctc cgctcgaagt agcgcgtctg ctgctccata caagccaacc
      601 acggcctcca gaagaagatg ttggcgacct cgtattggga atccccgaac atcgcctcgc
      661 tccagtcaat gaccgctgtt atgcggccat tgtccgtcag gacattgttg gagccgaaat
      721 ccgcgtgcac gaggtgccgg acttcggggc agtcctcggc ccaaagcatc agctcatcga
      781 gagcctgcgc gacggacgca ctgacggtgt cgtccatcac agtttgccag tgatacacat
      841 ggggatcagc aatcgcgcat atgaaatcac gccatgtagt gtattgaccg attccttgcg
      901 gtccgaatgg gccgaacccg ctcgtctggc taagatcggc cgcagcgatc gcatccatgg
      961 cctccgcgac cggctgcaga acagcgggca gttcggtttc aggcaggtct tgcaacgtga
     1021 caccctgtgc acggcggg
//
