LOCUS       sequence-72699-e         851 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the DHFR tag 
            by homologous recombination and selection for hygromycin 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 851)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH2
FEATURES             Location/Qualifiers
     source          1..851
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             37..516
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
                     TWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFL
                     PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatga tcagtctgat tgcggcgtta
       61 gcggtagatc gcgttatcgg catggaaaac gccatgccgt ggaacctgcc tgccgatctc
      121 gcctggttta aacgcaacac cttaaataaa cccgtgatta tgggccgcca tacctgggaa
      181 tcaatcggtc gtccgttgcc aggacgcaaa aatattatcc tcagcagtca accgggtacg
      241 gacgatcgcg taacgtgggt gaagtcggtg gatgaagcca tcgcggcgtg tggtgacgta
      301 ccagaaatca tggtgattgg cggcggtcgc gtttatgaac agttcttgcc aaaagcgcaa
      361 aaactgtatc tgacgcatat cgacgcagaa gtggaaggcg acacccattt cccggattac
      421 gagccggatg actgggaatc ggtattcagc gaattccacg atgctgatgc gcagaactct
      481 cacagctatt gctttgagat tctggagcgg cggtaacatc atcatcatca ttaactcgag
      541 cagctgaagc ttgcatgcct gcaggtcgac tctagaggat ccccgggtta attaaggcgc
      601 gccagatctg tttagcttgc ctcgtccccg ccgggtcacc cggccagcga catggaggcc
      661 cagaataccc tccttgacag tcttgacgtg cgcagctcag gggcatgatg tgactgtcgc
      721 ccgtacattt agcccataca tccccatgta taatcatttg catccataca ttttgatggc
      781 cgcacggcgc gaagcaaaaa ttacggctcc tcgctgcgga cctgcgagca gggaaacgct
      841 cccctcacag a
//
