LOCUS       sequence-72702-e        1054 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FLAG-intergenic region of TUB-HYG (modified from PMID 16269191).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1054)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pDS69
FEATURES             Location/Qualifiers
     source          1..1054
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(471..494)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(644..662)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(645..662)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(680..696)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(680..696)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(693..715)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    704..720
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(728..734,735..752,753..758))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    773..794
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(911..928)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
       61 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcactagt tctagatcaa
      121 gcttaagcta gcaattcgtt tgaactattt tctttgatga aagggttact attctttctc
      181 ctctagtagg gctggtcgag aaaacaagat tgaaagaaaa ggcggaaagg gagagggaga
      241 aagaggtgtg acacgacagg aagaagcgaa taaggggaag gaaggcatac acgcaaccac
      301 agacgtgaca gggtgcctcc tacacaaagg gtgagcaaaa aaacaagaaa aacataggtc
      361 ccaagcgaat taaaaacatt tgagacagaa aacaaattca caaaagaaag aaagaaagaa
      421 cataaatgaa aacctacaca tggtgcgacg ttgtcacact ttgtcgacta cttatcgtca
      481 tcgtccttgt aatcaatatc gtgatccttg taatcaccat cgtgatcctt gtactcgagg
      541 gggggcccgg taccagatct aaaaatcact agtgaattcg cggccgcctg caggtcgacc
      601 atatgggaga gctcccaacg cgttggatgc atagcttgag tattctatag tgtcacctaa
      661 atagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
      721 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
      781 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
      841 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
      901 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
      961 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     1021 acatgtgagc aaaaggccag caaaaggcca ggaa
//
