LOCUS       sequence-72704-e         816 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the DHFR tag 
            (c-terminal histag) by homologous recombination and selection for 
            phleomycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 816)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH3
FEATURES             Location/Qualifiers
     source          1..816
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(28..61)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(124..321)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ggccactagt ggatctgata tcacctaata acttcgtata gcatacatta tacgaagtta
       61 tattaagggt tctcgagagc tcgttttcga cactggatgg cggcgttagt atcgaatcga
      121 cagcagtata gcgaccagca ttcacatacg attgacgcat gatattactt tctgcgcact
      181 taacttcgca tctgggcaga tgatgtcgag gcgaaaaaaa atataaatca cgctaacatt
      241 tgattaaaat agaacaacta caatataaaa aaactataca aatgacaagt tcttgaaaac
      301 aagaatcttt ttattgtcag tactgatcat gagatgcctg caagcaattc gttctgtatc
      361 aggcgcagga gcgtcccgtc cgggtcgacc aaagcggcca tcgtgcctcc ccactcctgc
      421 agttcggggg catggatgcg cggatagccg ctgctggttt cctggatgcc gacggatttg
      481 cactgccggt agaactccgc gaggtcgtcc agcctcaggc agcagctgaa ccaactcgcg
      541 aggggatcga gcccggggtg ggcgaagaac tccagcatga gatccccgcg ctggaggatc
      601 atccagccgg cgtcccggaa aacgattccg aagcccaacc tttcatagaa ggcggcggtg
      661 ggatcgaaat ctcgtgatgg caggttgggc gtcgcttggt cggtcatacc catggttgtt
      721 tatgttcgga tgtgatgtga gaactgtatc ctagcaagat tttaaaagga agtatatgaa
      781 agaagaacct cagtggcaaa tcctaacctt ttatat
//
