LOCUS       sequence-72705-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the DHFR tag 
            by homologous recombination and selection for phleomycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH4
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(33..66)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     terminator      complement(129..326)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 gcataggcca ctagtggatc tgatatcacc taataacttc gtatagcata cattatacga
       61 agttatatta agggttctcg agagctcgtt ttcgacactg gatggcggcg ttagtatcga
      121 atcgacagca gtatagcgac cagcattcac atacgattga cgcatgatat tactttctgc
      181 gcacttaact tcgcatctgg gcagatgatg tcgaggcgaa aaaaaatata aatcacgcta
      241 acatttgatt aaaatagaac aactacaata taaaaaaact atacaaatga caagttcttg
      301 aaaacaagaa tctttttatt gtcagtactg atcatgagat gcctgcaagc aattcgttct
      361 gtatcaggcg caggagcgtc ccgtccgggt cgaccaaagc ggccatcgtg cctccccact
      421 cctgcagttc gggggcatgg atgcgcggat agccgctgct ggtttcctgg atgccgacgg
      481 atttgcactg ccggtagaac tccgcgaggt cgtccagcct caggcagcag ctgaaccaac
      541 tcgcgagggg atcgagcccg gggtgggcga agaactccag catgagatcc ccgcgctgga
      601 ggatcatcca gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg
      661 cggtgggatc gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atacccatgg
      721 ttgtttatgt tcggatgtga tgtgagaact gtatcctagc aagat
//
