LOCUS       sequence-72706-e         944 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the SNAP tag 
            by homologous recombination and selection for hygromycin 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 944)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 944)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH5
FEATURES             Location/Qualifiers
     source          1..944
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             22..44
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             52..597
                     /codon_start=1
                     /product="human O6-alkylguanine-DNA-alkyltransferase 
                     variant that forms covalent bonds with benzylguanine 
                     derivatives"
                     /label=SNAP-tag(R)
                     /note="optimized for bacterial expression"
                     /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAVE
                     VPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKLLK
                     VVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEGGLA
                     VKEWLLAHEGHRLGKPGLG"
ORIGIN
        1 cggtttccct ctagaaataa ttttgtttaa ctttaagaag gagatataca tatggacaaa
       61 gattgcgaaa tgaaacgtac caccctggat agcccgctgg gcaaactgga actgagcggc
      121 tgcgaacagg gcctgcatga aattaaactg ctgggtaaag gcaccagcgc ggccgatgcg
      181 gttgaagttc cggccccggc cgccgtgctg ggtggtccgg aaccgctgat gcaggcgacc
      241 gcgtggctga acgcgtattt tcatcagccg gaagcgattg aagaatttcc ggttccggcg
      301 ctgcatcatc cggtgtttca gcaggagagc tttacccgtc aggtgctgtg gaaactgctg
      361 aaagtggtta aatttggcga agtgattagc tatcagcagc tggcggccct ggcgggtaat
      421 ccggcggcca ccgccgccgt taaaaccgcg ctgagcggta acccggtgcc gattctgatt
      481 ccgtgccatc gtgtggttag ctctagcggt gcggttggcg gttatgaagg tggtctggcg
      541 gtgaaagagt ggctgctggc ccatgaaggt catcgtctgg gtaaaccggg tctgggacct
      601 gcaggtatag gcgcgccagg atccccgggt taattaaggc gcgccagatc tgtttagctt
      661 gcctcgtccc cgccgggtca cccggccagc gacatggagg cccagaatac cctccttgac
      721 agtcttgacg tgcgcagctc aggggcatga tgtgactgtc gcccgtacat ttagcccata
      781 catccccatg tataatcatt tgcatccata cattttgatg gccgcacggc gcgaagcaaa
      841 aattacggct cctcgctgcg gacctgcgag cagggaaacg ctcccctcac agacgcgttg
      901 aattgtcccc acgccgcgcc cctgtagaga aatataaaag gtta
//
