LOCUS       sequence-72708-e        1049 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the SNAP tag 
            by homologous recombination and selection for hygromycin 
            resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 1049)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH5
FEATURES             Location/Qualifiers
     source          1..1049
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(116..313)
                     /label=TEF terminator
                     /note="Ashbya gossypii TEF terminator"
ORIGIN
        1 ttcgggcttt gttagcagcc ggatctgctc gagcggccgc cagtgtgatg gatatcatcg
       61 atgaattcga gctcgttttc gacactggat ggcggcgtta gtatcgaatc gacagcagta
      121 tagcgaccag cattcacata cgattgacgc atgatattac tttctgcgca cttaacttcg
      181 catctgggca gatgatgtcg aggcgaaaaa aaatataaat cacgctaaca tttgattaaa
      241 atagaacaac tacaatataa aaaaactata caaatgacaa gttcttgaaa acaagaatct
      301 ttttattgtc agtactgatt attcctttgc cctcggacga gtgctggggc gtcggtttcc
      361 actatcggcg agtacttcta cacagccatc ggtccagacg gccgcgcttc tgcgggcgat
      421 ttgtgtacgc ccgacagtcc cggctccgga tcggacgatt gcgtcgcatc gaccctgcgc
      481 ccaagctgca tcatcgaaat tgccgtcaac caagctctga tagagttggt caagaccaat
      541 gcggagcata tacgcccgga gccgcggcga tcctgcaagc tccggatgcc tccgctcgaa
      601 gtagcgcgtc tgctgctcca tacaagccaa ccacggcctc cagaagaaga tgttggcgac
      661 ctcgtattgg gaatccccga acatcgcctc gctccagtca atgaccgctg ttatgcggcc
      721 attgtccgtc aggacattgt tggagccgaa atccgcgtgc acgaggtgcc ggacttcggg
      781 gcagtcctcg gcccaaagca tcagctcatc gagagcctgc gcgacggacg cactgacggt
      841 gtcgtccatc acagtttgcc agtgatacac atggggatca gcaatcgcgc atatgaaatc
      901 acgccatgta gtgtattgac cgattccttg cggtccgaat gggccgaacc cgctcgtctg
      961 gctaagatcg gccgcagcga tcgcatccat ggcctccgcg accggctgca gaacagcggg
     1021 cagttcggtt tcaggcaggt cttgcaacg
//
