LOCUS       sequence-72717-e         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial ST1 repression YFP reporter: protospacer A.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: SK-YFP-ST1-A
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     198..216
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(397..425)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccgacgt ctaagaaacc attattatca
      181 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcac ctcgagggga
      241 tccgataaat atctaacacc gtgcgtgttg actattttac ctctggcggt gataatggtt
      301 gcatgtacta gaattctttt caacaagctt gagatggtac tttaacttta agaaggagat
      361 atacatatgc gtaaaggaga agaacttttc actggagttg tcccaattct tgttgaatta
      421 gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg tgatgcaaca
      481 tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt tccatggcca
      541 acacttgtca ctactttcgg ttatggtcta aaatgctttg ctagataccc agatcatatg
      601 aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga aagaactata
      661 tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga aggtgatacc
      721 cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa cattcttgga
      781 cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga caaacaaaag
      841 aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag cgttcaacta
      901 gcagaccatt
//
