LOCUS       sequence-72718-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3XHA-intergenic region of TUB-HYG (modified from PMID 16269191).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pDS66
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(471..551)
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     regulatory      597..606
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      601..610
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(640..659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(641..659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(666..683)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(666..682)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(675..697)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(910..929)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
       61 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcactagt tctagatcaa
      121 gcttaagcta gcaattcgtt tgaactattt tctttgatga aagggttact attctttctc
      181 ctctagtagg gctggtcgag aaaacaagat tgaaagaaaa ggcggaaagg gagagggaga
      241 aagaggtgtg acacgacagg aagaagcgaa taaggggaag gaaggcatac acgcaaccac
      301 agacgtgaca gggtgcctcc tacacaaagg gtgagcaaaa aaacaagaaa aacataggtc
      361 ccaagcgaat taaaaacatt tgagacagaa aacaaattca caaaagaaag aaagaaagaa
      421 cataaatgaa aacctacaca tggtgcgacg ttgtcacact ttgtcgacta tgcgtaatcg
      481 ggcacatcgt acgggtatgc gtagtctggc acgtcgtatg ggtacgcgta atcaggcaca
      541 tcgtaagggt actcgagggg gggcccggta ccagatctaa aaatcgaatt cccgcggccg
      601 ccatggcggc cgggagcatg cgacgtcggg cccaattcgc cctatagtga gtcgtattac
      661 aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
      721 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
      781 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc tgtagcggcg
      841 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
      901 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
      961 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     1021 acc
//
