LOCUS       sequence-72722-e         677 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian tdTomato activation reporter for SP with 
            GTCCCCTCCACCCCACAGTG protospacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 677)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-tdTom-SP
FEATURES             Location/Qualifiers
     source          1..677
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        135..173
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     170..194
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      208..217
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             217..240
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(398..416)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctatagggcg aattgggccc tctagatgca tgctcgagcg gccgccagtg tgatggatat
       61 ctgcagaatt cgcccttgcg acgtagggat aacagggtaa tagtgtcccc tccaccccac
      121 agtgcgggaa acgaggtagg cgtgtacggt gggaggccta tataagcaga gctcgtttag
      181 tgaaccgtca gatcgcctgg agaattcgcc accatggact acaaggatga cgacgataaa
      241 acttccggtg gcggactggg ttccaccgtg agcaagggcg aggaggtcat caaagagttc
      301 atgcgcttca aggtgcgcat ggagggctcc atgaacggcc acgagttcga gatcgagggc
      361 gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggc
      421 ggccccctgc ccttcgcctg ggacatcctg tccccccagt tcatgtacgg ctccaaggcg
      481 tacgtgaagc accccgccga catccccgat tacaagaagc tgtccttccc cgagggcttc
      541 aagtgggagc gcgtgatgaa cttcgaggac ggcggtctgg tgaccgtgac ccaggactcc
      601 tccctgcagg acggcacgct gatctacaag gtgaagatgc gcggcaccaa cttccccccc
      661 gacggccccg taatgca
//
