LOCUS       sequence-72724-e        1073 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Hygromycin/Gancyclovir selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1073)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ17
FEATURES             Location/Qualifiers
     source          1..1073
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      187..196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgatgcgacg caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc
       61 agaagcgcgg ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga
      121 cgccccagca ctcgtccgag ggcaaaggaa tctagagaag tccatactaa ccaggaccca
      181 cttgacgcca ccatggcctc gtaccccggc catcaacacg cgtctgcgtt cgaccaggct
      241 gcgcgttctc gcggccatag caaccgacgt acggcgttgc gccctcgccg gcagcaagaa
      301 gccacggaag tccgcccgga gcagaaaatg cccacgctac tgcgggttta tatagacggt
      361 ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg ttcgcgcgac
      421 gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc ttccgagaca
      481 atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc ggccggggac
      541 gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc cgtgaccgac
      601 gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgcccc gcccccggcc
      661 ctcaccctcc tcctcgaccg ccatcccatc gccgtcatgc tgtgctaccc ggccgcgcgg
      721 taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct catcccgccg
      781 accttgcccg gcaccaacat cgtgcttggg gcccttccgg aggacagaca catcgaccgc
      841 ctggccaaac gccagcgccc cggcgagcgg ctggacctgg ctatgctggc tgcgattcgc
      901 cgcgtttacg ggctacttgc caatacggtg cggtatctgc agtgcggcgg gtcgtggcgg
      961 gaggactggg gacagctttc ggggacggcc gtgccgcccc agggtgccga gccccagagc
     1021 aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg ggc
//
