LOCUS       sequence-72729-e         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET29a_HHH CODA HIV integrase with C-terminal HIS tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Qi X, Vargas E, Larsen L, Knapp W, Hatfield GW, Lathrop R, Sandmeyer
            S
  TITLE     Directed DNA shuffling of retrovirus and retrotransposon integrase 
            protein domains.
  JOURNAL   PLoS One. 2013 May 17;8(5):e63957. doi: 
            10.1371/journal.pone.0063957. Print 2013.
  PUBMED    23691126
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-17"; volume: "8(5):e63957. doi"; pages: " 
            10.1371/journal.pone.0063957. Print 2013"
COMMENT     Sequence Label: pLZL2372
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             907..924
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgttcc tggatggcat cgataaagct
       61 caggaagaac acgaaaaata ccactctaac tggcgtgcaa tggcttctga tttcaacttg
      121 ccacctgttg ttgcaaaaga aatcgttgct tcctgcgaca aatgtcaact gaagggtgaa
      181 gctatgcacg gtcaggttga ttgctctcca ggtatttggc agttggattg cactcacctg
      241 gaagggaaag taattctggt tgctgttcac gtagcttctg gttacattga agctgaggtt
      301 attccggctg aaactggtca ggaaactgct tatttcttgc tgaaattggc aggtcgctgg
      361 ccggttaaaa ctgttcacac tgataacggt tccaacttca cttctactac tgttaaagct
      421 gcatgttggt gggctggtat caaacaggaa tttggtattc catacaaccc acagtcccaa
      481 ggcgttattg aatctatgaa caaagagctg aaaaagatta ttggtcaagt tcgtgatcag
      541 gctgaacacc tgaaaactgc tgtgcagatg gcagtattca ttcacaactt caaacgtaaa
      601 ggtggtattg gtggttacag cgcaggtgaa cgcattgttg atattattgc aactgacatt
      661 caaaccaagg aattgcagaa acagattacc aagattcaga acttccgtgt ttactaccgt
      721 gattctcgtg atccagtttg gaaaggccca gctaaactgt tgtggaaagg tgaaggtgct
      781 gttgttatcc aggataactc tgatattaaa gttgttccac gtcgtaaagc taaaatcatt
      841 cgtgattacg gtaaacagat ggctggtgat gattgtgttg cttctcgtca ggatgaagat
      901 ctcgagcacc accaccacca ccactgagat ccggctgcta acaaagcccg aaaggaagct
      961 gagttggctg ctgccaccgc tgagca
//
