LOCUS       sequence-7273-ex       12168 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12168)
  TITLE     Trono Lab Constitutive Lentiviral Plasmids
REFERENCE   2  (bases 1 to 12168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pWPT-nlsLacZ
FEATURES             Location/Qualifiers
     source          1..12168
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             2..635
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    682..807
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1304..1537
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1722..1766
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2033..2150
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2206..2417
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     primer_bind     complement(2627..2644)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2627..2643)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2634..5681
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(2636..2658)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    5973..6561
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6026..6046)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6444..6455)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     protein_bind    complement(6730..6763)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     LTR             6783..6963
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(6988..7006)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(6989..7006)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(7296..7315)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(7529..7548)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7648..7670
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7708..7726)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7794..7898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7899..7967,7968..8759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8117..8136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8930..9518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9419..9438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9672..9689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(9759..9779)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9764..10093
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9944..10079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          11227..11292
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             11422..11442
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    11867..12001
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11904..11923)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11958..11977
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg gatctaccac
       61 acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt cagatatcca
      121 ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt agaagaggcc
      181 aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      241 gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca cgtggcccga
      301 gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa gggactttcc
      361 gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg agccctcaga
      421 tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag accagatctg
      481 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
      541 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
      601 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
      661 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
      721 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
      781 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
      841 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
      901 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
      961 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     1021 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     1081 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     1141 caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa
     1201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
     1261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
     1321 ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg
     1381 ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg
     1441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc
     1501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
     1561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
     1621 agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct
     1681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
     1741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
     1801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
     1861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
     1921 tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
     1981 acagagacag atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa
     2041 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     2101 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt ccgatcacga
     2161 gactagcctc gagaagcttg atcgatggct ccggtgcccg tcagtgggca gagcgcacat
     2221 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     2281 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     2341 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     2401 ttgccgccag aacacaggtg tcgtgacgcg gatcgatcct ctagagtcga cggcctctga
     2461 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttg
     2521 caaagatgga taaagttttc cggaattccg caaaaaagaa gagaaaggta gaagacccca
     2581 aggactttcc ttcagaattg ctaagttttt tgagtccaag cttggcactg gccgtcgttt
     2641 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     2701 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     2761 tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
     2821 aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
     2881 agatgcacgg ttacgatgcg cccatctaca ccaacgtgac ctatcccatt acggtcaatc
     2941 cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
     3001 aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttaac tcggcgtttc
     3061 atctgtggtg caacgggcgc tgggtcggtt acggccagga cagtcgtttg ccgtctgaat
     3121 ttgacctgag cgcattttta cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt
     3181 ggagtgacgg cagttatctg gaagatcagg atatgtggcg gatgagcggc attttccgtg
     3241 acgtctcgtt gctgcataaa ccgactacac aaatcagcga tttccatgtt gccactcgct
     3301 ttaatgatga tttcagccgc gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc
     3361 gtgactacct acgggtaaca gtttctttat ggcagggtga aacgcaggtc gccagcggca
     3421 ccgcgccttt cggcggtgaa attatcgatg agcgtggtgg ttatgccgat cgcgtcacac
     3481 tacgtctgaa cgtcgaaaac ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg
     3541 cggtggttga actgcacacc gccgacggca cgctgattga agcagaagcc tgcgatgtcg
     3601 gtttccgcga ggtgcggatt gaaaatggtc tgctgctgct gaacggcaag ccgttgctga
     3661 ttcgaggcgt taaccgtcac gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga
     3721 cgatggtgca ggatatcctg ctgatgaagc agaacaactt taacgccgtg cgctgttcgc
     3781 attatccgaa ccatccgctg tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg
     3841 atgaagccaa tattgaaacc cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc
     3901 gctggctacc ggcgatgagc gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc
     3961 cgagtgtgat catctggtcg ctggggaatg aatcaggcca cggcgctaat cacgacgcgc
     4021 tgtatcgctg gatcaaatct gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag
     4081 ccgacaccac ggccaccgat attatttgcc cgatgtacgc gcgcgtggat gaagaccagc
     4141 ccttcccggc tgtgccgaaa tggtccatca aaaaatggct ttcgctacct ggagagacgc
     4201 gcccgctgat cctttgcgaa tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta
     4261 aatactggca ggcgtttcgt cagtatcccc gtttacaggg cggcttcgtc tgggactggg
     4321 tggatcagtc gctgattaaa tatgatgaaa acggcaaccc gtggtcggct tacggcggtg
     4381 attttggcga tacgccgaac gatcgccagt tctgtatgaa cggtctggtc tttgccgacc
     4441 gcacgccgca tccagcgctg acggaagcaa aacaccagca gcagtttttc cagttccgtt
     4501 tatccgggca aaccatcgaa gtgaccagcg aatacctgtt ccgtcatagc gataacgagc
     4561 tcctgcactg gatggtggcg ctggatggta agccgctggc aagcggtgaa gtgcctctgg
     4621 atgtcgctcc acaaggtaaa cagttgattg aactgcctga actaccgcag ccggagagcg
     4681 ccgggcaact ctggctcaca gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag
     4741 ccgggcacat cagcgcctgg cagcagtggc gtctggcgga aaacctcagt gtgacgctcc
     4801 ccgccgcgtc ccacgccatc ccgcatctga ccaccagcga aatggatttt tgcatcgagc
     4861 tgggtaataa gcgttggcaa tttaaccgcc agtcaggctt tctttcacag atgtggattg
     4921 gcgataaaaa acaactgctg acgccgctgc gcgatcagtt cacccgtgca ccgctggata
     4981 acgacattgg cgtaagtgaa gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga
     5041 aggcggcggg ccattaccag gccgaagcag cgttgttgca gtgcacggca gatacacttg
     5101 ctgatgcggt gctgattacg accgctcacg cgtggcagca tcaggggaaa accttattta
     5161 tcagccggaa aacctaccgg attgatggta gtggtcaaat ggcgattacc gttgatgttg
     5221 aagtggcgag cgatacaccg catccggcgc ggattggcct gaactgccag ctggcgcagg
     5281 tagcagagcg ggtaaactgg ctcggattag ggccgcaaga aaactatccc gaccgcctta
     5341 ctgccgcctg ttttgaccgc tgggatctgc cattgtcaga catgtatacc ccgtacgtct
     5401 tcccgagcga aaacggtctg cgctgcggga cgcgcgaatt gaattatggc ccacaccagt
     5461 ggcgcggcga cttccagttc aacatcagcc gctacagtca acagcaactg atggaaacca
     5521 gccatcgcca tctgctgcac gcggaagaag gcacatggct gaatatcgac ggtttccata
     5581 tggggattgg tggcgacgac tcctggagcc cgtcagtatc ggcggaattc cagctgagcg
     5641 ccggtcgcta ccattaccag ttggtctggt gtcaaaaata ataataaccg ggcaggccat
     5701 gtctgcccgt atttcgcgta aggaaatcca ttatgtacta tttaaaaaac acaaactttt
     5761 ggatgttcgg tttattcttt ttcttttact tttttatcat gggagcctac ttcccgtttt
     5821 tcccgatttg gctacatgac atcaaccata tcagcaaaag tgatacgggt attatttttg
     5881 ccgctatttc tctgttctcg ctattattcc aaccgctgtt tggtctgctt tctgacaaac
     5941 tcggcggatc tcgacctcga gggaattccg ataatcaacc tctggattac aaaatttgtg
     6001 aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt
     6061 taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata
     6121 aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg
     6181 tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc
     6241 tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct
     6301 gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt
     6361 cggggaagct gacgtccttt ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg
     6421 ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc
     6481 tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct
     6541 ccctttgggc cgcctccccg catcgggaat tcgctcaagc ttcgaattaa ttctgcagag
     6601 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     6661 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatg ggatcaattc
     6721 accatgggaa taacttcgta tagcatacat tatacgaagt tatgctgctt tttgcttgta
     6781 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     6841 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     6901 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     6961 gcagcatcta gaattaattc cgtgtattct atagtgtcac ctaaatcgta tgtgtatgat
     7021 acataaggtt atgtattaat tgtagccgcg ttctaacgac aatatgtaca agcctaattg
     7081 tgtagcatct ggcttactga agcagaccct atcatctctc tcgtaaactg ccgtcagagt
     7141 cggtttggtt ggacgaacct tctgagtttc tggtaacgcc gtcccgcacc cggaaatggt
     7201 cagcgaacca atcagcaggg tcatcgctag ccagatcctc tacgccggac gcatcgtggc
     7261 cggcatcacc ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg
     7321 ggaagatcgg gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc
     7381 aggccccgtg gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc
     7441 ggcggtgctc aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa
     7501 gggagagcgt cgaatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc
     7561 agccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat
     7621 ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt
     7681 catcaccgaa acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg
     7741 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa
     7801 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
     7861 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
     7921 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
     7981 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
     8041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     8101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     8161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     8221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     8281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     8341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     8401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     8461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     8521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     8581 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
     8641 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
     8701 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
     8761 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
     8821 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
     8881 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
     8941 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
     9001 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
     9061 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
     9121 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
     9181 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
     9241 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
     9301 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
     9361 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
     9421 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
     9481 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt
     9541 tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg
     9601 attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa
     9661 cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc
     9721 ctctccccgc gcgttggccg attcattaat gcagctgtgg aatgtgtgtc agttagggtg
     9781 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     9841 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     9901 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     9961 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
    10021 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
    10081 aggcttttgc aaaaagcttg gacacaagac aggcttgcga gatatgtttg agaataccac
    10141 tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg
    10201 tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg aacacttttt
    10261 aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc gtcacctggg
    10321 acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct gaacaatgga
    10381 aaggcattat tgccgtaagc cgtggcggtc tgtaccgggt gcgttactgg cgcgtgaact
    10441 gggtattcgt catgtcgata ccgtttgtat ttccagctac gatcacgaca accagcgcga
    10501 gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc ttcatcgtta ttgatgacct
    10561 ggtggatacc ggtggtactg cggttgcgat tcgtgaaatg tatccaaaag cgcactttgt
    10621 caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat gactatgttg ttgatatccc
    10681 gcaagatacc tggattgaac agccgtggga tatgggcgtc gtattcgtcc cgccaatctc
    10741 cggtcgctaa tcttttcaac gcctggcact gccgggcgtt gttcttttta acttcaggcg
    10801 ggttacaata gtttccagta agtattctgg aggctgcatc catgacacag gcaaacctga
    10861 gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac ctcgacgcta gtccgccgct
    10921 ttaatcacgg cgcacaaccg cctgtgcagt cggcccttga tggtaaaacc atccctcact
    10981 ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg cattgaccca cgcgaaatcc
    11041 tcgacgtcca ggcacgtatt gtgatgagcg atgccgaacg taccgacgat gatttatacg
    11101 atacggtgat tggctaccgt ggcggcaact ggatttatga gtgggccccg gatctttgtg
    11161 aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc
    11221 tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt
    11281 tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt
    11341 aatgaggaaa acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct
    11401 gactctcaac attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt
    11461 ccttcagaat tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc
    11521 tttgctattt acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa
    11581 tattctgtaa cctttataag taggcataac agttataatc ataacatact gttttttctt
    11641 actccacaca ggcatagagt gtctgctatt aataactatg ctcaaaaatt gtgtaccttt
    11701 agctttttaa tttgtaaagg ggttaataag gaatatttga tgtatagtgc cttgactaga
    11761 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
    11821 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
    11881 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
    11941 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
    12001 caactggata actcaagcta accaaaatca tcccaaactt cccaccccat accctattac
    12061 cactgccaat tacctagtgg tttcatttac tctaaacctg tgattcctct gaattatttt
    12121 cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtag
//
