LOCUS       sequence-72731-e        1011 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pET29a_PPP CODA Ty3 integrase with C-terminal His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Qi X, Vargas E, Larsen L, Knapp W, Hatfield GW, Lathrop R, Sandmeyer
            S
  TITLE     Directed DNA shuffling of retrovirus and retrotransposon integrase 
            protein domains.
  JOURNAL   PLoS One. 2013 May 17;8(5):e63957. doi: 
            10.1371/journal.pone.0063957. Print 2013.
  PUBMED    23691126
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-05-17"; volume: "8(5):e63957. doi"; pages: " 
            10.1371/journal.pone.0063957. Print 2013"
COMMENT     Sequence Label: pLZL2374
FEATURES             Location/Qualifiers
     source          1..1011
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatgacta tcactccaga aacttctcgc
       61 ccgattgata ccgagtcttg gaaatcttac tacaaatctg atccactgtg ctctgccgtt
      121 ctgattcaca tgaaagaatt gactcagcac aacgttactc cggaagatat gtctgctttc
      181 cgttcttacc agaagaaact ggaactgtct gaaactttcc gcaaaaacta ctctctggaa
      241 gatgaaatga tctactacca ggatcgtctg gttgttccga ttaaacagca gaacgcagtt
      301 atgcgcttgt accacgatca cactctgttc ggcggtcact tcggtgttac tgttactctg
      361 gctaagattt ctccaattta ctactggcca aaattgcagc actccattat tcagtacatt
      421 cgtacttgcg ttcagtgcca actgattaaa tctcaccgtc cacgtcttca cggtctgttg
      481 cagccactgc caattgccga aggccgttgg ctggacattt ctatggattt cgttactggt
      541 ctgccaccaa cttctaacaa cctgaacatg atcctcgttg ttgttgatcg tttctctaaa
      601 cgtgctcact tcattgctac tcgtaaaact ttggatgcta ctcaactgat cgatctgctg
      661 ttccgttaca ttttctctta ccacggtttc ccacgtacta ttacttctga tcgtgatgtt
      721 cgtatgactg ctgataaata ccaggaactg actaaacgcc tgggtattaa atccactatg
      781 tcttctgcta accacccaca gactgatggt cagtccgaac gtaccattca gaccctgaac
      841 cgtttgctgc gtgcttacgc ttctaccaac atccagaact ggcacgttta cctgccacag
      901 attgagtttg tttacaactc cactccaact cgtaccctgg gtaaatctcc attcgaaatt
      961 gatctgggtt acttgccaaa cactccagct atcaagtctg atgatgaagt t
//
