LOCUS       sequence-72732-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian tdTomato activation reporter for ST1 with 
            GTCCCCTCCACCCCACAGTG protospacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-tdTom-ST1
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     110..127
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    138..219
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     163..182
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(502..525)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(700..719)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtaccgagct cggatccact agtaacggcc gccagtgtgc tggaattcgc cctttattac
       61 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
      121 gagcgaggaa gctcgaggca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt
      181 gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat
      241 tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac
      301 aaatgtggta tggctgatta tgatcctcta gagatatcgt cgacaagctt atcgatgcgg
      361 ccgcgctagc acgcgtcagc tgactagagg atccccgggt accgagctcg aattcttact
      421 tgtacagctc gtccatgccg tacaggaaca ggtggtggcg gccctcggag cgctcgtact
      481 gttccacgat ggtgtagtcc tcgttgtggg aggtgatgtc cagcttggtg tccacgtagt
      541 agtagccggg cagttgcacg ggcttcttgg ccatgtagat ggtcttgaac tccaccaggt
      601 agtggccgcc gtccttcagc ttcagggcct ggtggatctc gcccttcagc acgccgtcgc
      661 gggggtacag gcgctcggtg gaggcctccc agcccatggt cttcttctgc attacggggc
      721 cgtcgggggg gaagttggtg ccgcgcatct tcaccttgta gatcagcgtg ccgtcctgca
      781 gggaggagtc ctgggtcacg gtcaccagac cgccgtcctc gaagttcatc acgcgctccc
      841 acttgaagcc ctcggggaag gacagcttct tgtaatcggg gatgtcggcg gg
//
