LOCUS       sequence-72735-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian tdTomato activation reporter for SP with 
            GTCCCCTCCACCCCACAGTG protospacer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-tdTom-SP
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     106..123
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    134..215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     159..178
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(498..521)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(696..715)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 cgagctcgga tccactagta acggccgcca gtgtgctgga attcgccctt tattaccgcc
       61 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
      121 gaggaagctc gaggcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt
      181 tatttgtaac cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta
      241 tgtttcaggt tcagggggag gtgtgggagg ttttttaaag caagtaaaac ctctacaaat
      301 gtggtatggc tgattatgat cctctagaga tatcgtcgac aagcttatcg atgcggccgc
      361 gctagcacgc gtcagctgac tagaggatcc ccgggtaccg agctcgaatt cttacttgta
      421 cagctcgtcc atgccgtaca ggaacaggtg gtggcggccc tcggagcgct cgtactgttc
      481 cacgatggtg tagtcctcgt tgtgggaggt gatgtccagc ttggtgtcca cgtagtagta
      541 gccgggcagt tgcacgggct tcttggccat gtagatggtc ttgaactcca ccaggtagtg
      601 gccgccgtcc ttcagcttca gggcctggtg gatctcgccc ttcagcacgc cgtcgcgggg
      661 gtacaggcgc tcggtggagg cctcccagcc catggtcttc ttctgcatta cggggccgtc
      721 gggggggaag ttggtgccgc gcatcttcac cttgtagatc agcgtgccgt cctgcaggga
      781 ggagtcctgg gtcacggtca ccagaccgcc gtcctcgaag ttcatcacgc gctcccactt
      841 gaagccctcg gggaaggaca gcttcttgta atcggggatg tcg
//
