LOCUS       sequence-72738-e         599 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian NM-VP64 nuclease-null Cas9 activator expression, human 
            optimized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 599)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-NMn-VP64
FEATURES             Location/Qualifiers
     source          1..599
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..26
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      114..123
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gagctcgttt agtgaaccgt cagatcgcct ggagacgcca tccacgctgt tttgacctcc
       61 atagaagaca ccgggaccga tccagcctcc ggactctaga ggatcgaacc cttgccacca
      121 tggccgcctt caagcccaac cccatcaact acatcctggg cctggccatc ggcatcgcca
      181 gcgtgggctg ggccatggtg gagatcgacg aggacgagaa ccccatctgc ctgatcgacc
      241 tgggtgtgcg cgtgttcgag cgcgctgagg tgcccaagac tggtgacagt ctggctatgg
      301 ctcgccggct tgctcgctct gttcggcgcc ttactcgccg gcgcgctcac cgccttctgc
      361 gcgctcgccg cctgctgaag cgcgagggtg tgctgcaggc tgccgacttc gacgagaacg
      421 gcctgatcaa gagcctgccc aacactcctt ggcagctgcg cgctgccgct ctggaccgca
      481 agctgactcc tctggagtgg agcgccgtgc tgctgcacct gatcaagcac cgcggctacc
      541 tgagccagcg caagaacgag ggcgagaccg ccgacaagga gctgggtgct ctgctggaa
//
