LOCUS       sequence-72742-e        1005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Blasticidin/Gancyclovir selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ41
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    183..216
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gcttggccat gctgagatct gtaatgtcag tgcgagcgat caagaatgta acacaaagga
       61 ataccaagtt tgtgaatttt attttttggt gtaattgaag tcttcaagtt gaaaaaaatg
      121 gggaaaagga aaaaaaactc gaaggcgcgt gcagcggccg gccgacgaga acgtgtaccg
      181 gtataacttc gtataatgta tgctatacga agttataagc ttcgttgcag ttgagtttct
      241 gttttcgggc ttgttggtat acttgtttga ttaatgtggc tgatagctaa gctatcgcaa
      301 atatttgtac aagacatgag ggggcaaaaa aataagccaa gtgtatttgt taggcttccg
      361 tgcctgttag agaaatatgt aaggaaacgc gtgcgctaca cagcttgagc accctcgagc
      421 atcttcatta cctactgcta atgacgaacg ggaaatgccc cgtccgcgtc tagtattgct
      481 cctcctcgtc gaactcgccc tcctcctcaa tcgtggcatc ctggtactgc tggtactcag
      541 acacgagatc gttcatgttg gactctgcct ccgtgaattc catctcgtcc ataccctcgc
      601 cagtgtacca gtgcaagaac gccttgcgac ggaacatgag ggtgaactgc tctcccacac
      661 ggcggaacat ctcctggatg caggtgttgt tgccaatgaa ggtgacagcc atcttgagtc
      721 ccttgggtgg gatatcgcaa acagaggact tgatgttgtt cgggatctcc actcaatgaa
      781 gtaggacgag ttcttgttct gcacgttcag catctgctca tcaacctcct tcgtcgacat
      841 gcggccgcgg aagagtgcag acgctgtcag gtagcggccg tgacgaggat ctgcagcttg
      901 catcatgttt ttcgcatcga acatctgctg cgttagctcg ggcacggaga gaccgcggta
      961 ctgctgcgag ccgcggctgg tcagcggggc gaagcccatc atgaa
//
