LOCUS       sequence-72743-e         778 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV-mediated expression of ChR2-Venus for neuronal excitation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   
  TITLE     The subcellular organization of neocortical excitatory connections.
  JOURNAL   Nature. 2009 Jan 18.
  PUBMED    19151697
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2009 Jan 18."
COMMENT     Sequence Label: pACAGW-ChR2-Venus-AAV
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(82..101)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          84..216
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(245..496)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(287..308)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     636..655
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 tttcaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat tcgaatcgac
       61 tagtcgcgag ctccactcga cacctgtgga gagaaaggca aagtggatgt cagtaagacc
      121 aataggtgcc tatcagaaac gcaagagtct tctctgtctc gacaagccca gtttctattg
      181 gtctccttaa acctgtcttg taaccttgat acttacctgc ggcctcgagt ccggggccgc
      241 tttacttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
      301 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg tagctcaggt
      361 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
      421 cggcgagctg cacgccgccg tcctcgatgt tgtggcggat cttgaagttg gccttgatgc
      481 cgttcttctg cttgtcggcg gtgatataga cgttgtggct gttgtagttg tactccagct
      541 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
      601 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
      661 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
      721 gcttcatgtg gtcggggtag cgggcgaagc actgcaggcc gtagcccagg gtggtcac
//
