LOCUS       sequence-72745-e        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Hygromycin selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ32
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    287..320
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gagattcttc gccctccgag agctgcatca ggtcggagac gctgtcgaac ttttcgatca
       61 gaaacttctc gacagacgtc gcggtgagtt caggcttttt catcatttac caacagtaag
      121 atctgtaatg tcagtgcgag cgatcaagaa tgtaacacaa aggaatacca agtttgtgaa
      181 ttttattttt tggtgtaatt gaagtcttca agttgaaaaa aatggggaaa aggaaaaaaa
      241 actcgaaggc gcgtgcagcg gccggccgac gagaacgtgt accggtataa cttcgtataa
      301 tgtatgctat acgaagttat aagcttcgtt gcagttgagt ttctgttttc gggcttgttg
      361 gtatacttgt ttgattaatg tggctgatag ctaagctatc gcaaatattt gtacaagaca
      421 tgagggggca aaaaaataag ccaagtgtat ttgttaggct tccgtgcctg ttagagaaat
      481 atgtaaggaa acgcgtgcgc tacacagctt gagcaccctc gagcatcttc attacctact
      541 gctaatgacg aacgggaaat gccccgtccg cgtctagtat tgctcctcct cgtcgaactc
      601 gccctcctcc tcaatcgtgg catcctggta ctgctggtac tcagacacga gatcgttcat
      661 gttggactct gcctccgtga attccatctc gtccataccc tcgccagtgt accagtgcaa
      721 gaacgccttg cgacggaaca tgagggtgaa ctgctctccc acacggcgga acatctcctg
      781 gatgcaggtg ttgttgccaa tgaaggtgac agccatcttg agtcccttgg gtgggatatc
      841 gcaaacagag gacttgatgt tgttcgggat ctccactcaa tgaagtagga cgagttcttg
      901 ttctgcacgt tcagcatctg ctcatcaacc tccttcgtcg acatgcggcc gcggaagagt
      961 gcagacgctg tcaggtagcg gccgtgacga ggatctgcag cttgcatcat gtttttcgca
     1021 tcgaacatct gctgcgttag ctcgggcacg gagagaccgc ggtactgctg cgagccgcgg
     1081 ctggtc
//
