LOCUS       sequence-72753-e        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Targets beta-Tubulin for Cre-lox mediated knockout in T. brucei, 
            Puromycin selectable.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Kim HS, Li Z, Boothroyd C, Cross GA
  TITLE     Strategies to construct null and conditional null Trypanosoma brucei
            mutants using Cre-recombinase and loxP.
  JOURNAL   Mol Biochem Parasitol. 2013 Aug 13;191(1):16-19. doi: 
            10.1016/j.molbiopara.2013.08.001.
  PUBMED    23954366
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.molbiopara.2013.08"; journalName: "Mol Biochem 
            Parasitol"; date: "2013-08-13- 13"; volume: "191"; issue: "1"; 
            pages: "16-19"
COMMENT     Sequence Label: pHJ33
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    157..190
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cagtgcgagc gatcaaagaa tgtaacacaa aggaatacca agtttgtgaa ttttattttt
       61 tggtgtaatt gaagtcttca agttgaaaaa aatggggaaa aggaaaaaaa actcgaaggc
      121 gcgtgcagcg gccggccgac gagaacgtgt accggtataa cttcgtataa tgtatgctat
      181 acgaagttat aagcttcgtt gcagttgagt ttctgttttc gggcttgttg gtatacttgt
      241 ttgattaatg tggctgatag ctaagctatc gcaaatattt gtacaagaca tgagggggca
      301 aaaaaataag ccaagtgtat ttgttaggct tccgtgcctg ttagagaaat atgtaaggaa
      361 acgcgtgcgc tacacagctt gagcaccctc gagcatcttc attacctact gctaatgacg
      421 aacgggaaat gccccgtccg cgtctagtat tgctcctcct cgtcgaactc gccctcctcc
      481 tcaatcgtgg catcctggta ctgctggtac tcagacacga gatcgttcat gttggactct
      541 gcctccgtga attccatctc gtccataccc tcgccagtgt accagtgcaa gaacgccttg
      601 cgacggaaca tgagggtgaa ctgctctccc acacggcgga acatctcctg gatgcaggtg
      661 ttgttgccaa tgaaggtgac agccatcttg agtcccttgg gtgggatatc gcaaacagag
      721 gacttgatgt tgttcgggat ctccactcaa tgaagtagga cgagttcttg ttctgcacgt
      781 tcagcatctg ctcatcaacc tccttcgtcg acatgcggcc gcggaagagt gcagacgctg
      841 tcaggtagcg gccgtgacga ggatctgcag cttgcatcat gtttttcgca tcgaacatct
      901 gctgcgttag ctcgggcacg gagagaccgc ggtactgctg cgagccgcgg ctggtcagcg
      961 gggcgaagcc catcatgaag aagtgcagac gcgggaatgg gacaaggttc acagccaact
     1021 ta
//
