LOCUS       sequence-72755-e        1084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Cas9 (D10A) mutant nickase 
            and GFP, plasmid optimized for expression in human pluripotent stem 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1084)
  AUTHORS   Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K
  TITLE     Enhanced efficiency of human pluripotent stem cell genome editing 
            through replacing TALENs with CRISPRs.
  JOURNAL   Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 
            10.1016/j.stem.2013.03.006.
  PUBMED    23561441
REFERENCE   2  (bases 1 to 1084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.03"; journalName: "Cell Stem Cell"; date: 
            "2013-04-4- 4"; volume: "12"; issue: "4"; pages: "393-4"
COMMENT     Sequence Label: pCas9D10A_GFP
FEATURES             Location/Qualifiers
     source          1..1084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(113..124)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tcagccttag tcagattatc gaacttccgt tgtgtgatca gtttggcgtt cagcagctgc
       61 cgccaataat ttttcatttt cttgacaact tcttctgagg ggacgttatc actcttccct
      121 ctatttttat cggatcttgt caacacttta ttatcaatag aatcatcttt gagaaaagac
      181 tggggcacga tatgatccac gtcgtagtcg gagagccgat tgatgtccag ttcctgatcc
      241 acgtacatgt ccctgccgtt ctgcaggtag tacaggtaga gcttctcatt ctgaagctgg
      301 gtgttttcaa ctgggtgttc cttaaggatt tgggacccca gttcttttat accctcttca
      361 atcctcttca tcctttccct actgttcttc tgtcccttct gggtagtttg gttctctcgg
      421 gccatctcga taacgatatt ctcgggctta tgccttccca ttactttgac gagttcatcc
      481 acgaccttaa cggtctgcag tattcccttt ttgatagctg ggctacctgc aagattagcg
      541 atgtgctcgt gaagactgtc cccctggcca gaaacttgtg ctttctggat gtcctcctta
      601 aaggtgagag agtcatcatg gatcaactgc atgaagttcc ggttggcaaa tccatcggac
      661 ttaagaaaat ccaggattgt ctttccactc tgcttgtctc ggatcccatt gatcagtttt
      721 cttgacagcc gcccccatcc tgtatatcgg cgcctcttga gctgtttcat gactttgtcg
      781 tcgaagagat gagcgtaagt tttcaagcgt tcttcaatca tctccctatc ttcaaacaac
      841 gtaagggtga ggacaatgtc ctcaagaatg tcctcgttct cctcattgtc caggaagtcc
      901 ttgtctttaa tgattttcag gagatcgtga tacgttccca gggatgcgtt gaagcgatcc
      961 tccactccgc tgatttcaac agagtcgaaa cattcaatct ttttgaaata gtcttctttg
     1021 agctgtttca cggtaacttt ccggttcgtc ttgaagagga ggtccacgat agctttcttc
     1081 tgct
//
