LOCUS       sequence-72756-e         951 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Co-expression of human codon-optimized Cas9 (D10A) mutant nickase 
            and GFP, plasmid optimized for expression in human pluripotent stem 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Ding Q, Regan SN, Xia Y, Oostrom LA, Cowan CA, Musunuru K
  TITLE     Enhanced efficiency of human pluripotent stem cell genome editing 
            through replacing TALENs with CRISPRs.
  JOURNAL   Cell Stem Cell. 2013 Apr 4;12(4):393-4. doi: 
            10.1016/j.stem.2013.03.006.
  PUBMED    23561441
REFERENCE   2  (bases 1 to 951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stem.2013.03"; journalName: "Cell Stem Cell"; date: 
            "2013-04-4- 4"; volume: "12"; issue: "4"; pages: "393-4"
COMMENT     Sequence Label: pCas9D10A_GFP
FEATURES             Location/Qualifiers
     source          1..951
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      5..14
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggccgccacc atggacaaga agtactccat tgggctcgct atcggcacaa acagcgtcgg
       61 ctgggccgtc attacggacg agtacaaggt gccgagcaaa aaattcaaag ttctgggcaa
      121 taccgatcgc cacagcataa agaagaacct cattggcgcc ctcctgttcg actccgggga
      181 gacggccgaa gccacgcggc tcaaaagaac agcacggcgc agatataccc gcagaaagaa
      241 tcggatctgc tacctgcagg agatctttag taatgagatg gctaaggtgg atgactcttt
      301 cttccatagg ctggaggagt cctttttggt ggaggaggat aaaaagcacg agcgccaccc
      361 aatctttggc aatatcgtgg acgaggtggc gtaccatgaa aagtacccaa ccatatatca
      421 tctgaggaag aagcttgtag acagtactga taaggctgac ttgcggttga tctatctcgc
      481 gctggcgcat atgatcaaat ttcggggaca cttcctcatc gagggggacc tgaacccaga
      541 caacagcgat gtcgacaaac tctttatcca actggttcag acttacaatc agcttttcga
      601 agagaacccg atcaacgcat ccggagttga cgccaaagca atcctgagcg ctaggctgtc
      661 caaatcccgg cggctcgaaa acctcatcgc acagctccct ggggagaaga agaacggcct
      721 gtttggtaat cttatcgccc tgtcactcgg gctgaccccc aactttaaat ctaacttcga
      781 cctggccgaa gatgccaagc ttcaactgag caaagacacc tacgatgatg atctcgacaa
      841 tctgctggcc cagatcggcg accagtacgc agaccttttt ttggcggcaa agaacctgtc
      901 agacgccatt ctgctgagtg atattctgcg agtgaacacg gagatcacca a
//
