LOCUS       sequence-72761-e         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lacritin protein with 45 AA removed from N 
            terminus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler 
            MF 4th, Lee JK, Laurie GW
  TITLE     Lacritin rescues stressed epithelia via rapid forkhead box O3 
            (FOXO3)-associated autophagy that restores metabolism.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 
            10.1074/jbc.M112.436584. Epub 2013 May 2.
  PUBMED    23640897
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.436584"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18146-61"
COMMENT     Sequence Label: pTYB1-pLAC-N45
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggcggca gcagttcagg ggacagccaa
       61 ggtcacctca agcaggcagg aactaaaccc cctgaaatcc atagtggaga aaagtatctt
      121 actaacagaa caagcccttg caaaagcagg aaaaggaatg cacggaggcg tgccaggtgg
      181 aaaacaattc atcgaaaatg gaagtgaatt tgcacaaaaa ttactgaaga aattcagtct
      241 attaaaacca tgggcatgct ttgccaaggg taccaatgtt ttaatggcgg atgggtctat
      301 tgaatgtatt gaaaacattg aggttggtaa taaggtcatg ggtaaagatg gcagacctcg
      361 tgaggtaatt aaattgccca gaggaagaga aactatgtac agcgtcgtgc agaaaagtca
      421 gcacagagcc cacaaaagtg actcaagtcg tgaagtgcca gaattactca agtttacgtg
      481 taatgcgacc catgagttgg ttgttagaac acctcgtagt gtccgccgtt tgtctcgtac
      541 cattaagggt gtcgaatatt ttgaagttat tacttttgag atgggccaaa agaaagcccc
      601 cgacggtaga attgttgagc ttgtcaagga agtttcaaag agctacccaa tatctgaggg
      661 gcctgagaga gccaacgaat tagtagaatc ctatagaaag gcttcaaata aagcttattt
      721 tgagtggact attgaggcca gagatctttc tctgttgggt tcccatgttc gtaaagctac
      781 ctaccagact tacgctccaa ttctttatga gaatgaccac tttttcgact acatgcaaaa
      841 aagtaagttt catctcacca ttgaa
//
