LOCUS       sequence-72766-e         869 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lacritin Protein with Mutation at protein 73
            from Isoleucine to Serine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 869)
  AUTHORS   Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler 
            MF 4th, Lee JK, Laurie GW
  TITLE     Lacritin rescues stressed epithelia via rapid forkhead box O3 
            (FOXO3)-associated autophagy that restores metabolism.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 
            10.1074/jbc.M112.436584. Epub 2013 May 2.
  PUBMED    23640897
REFERENCE   2  (bases 1 to 869)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.436584"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18146-61"
COMMENT     Sequence Label: pTYB1-pLAC-I73S
FEATURES             Location/Qualifiers
     source          1..869
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             2..24
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttttgtttaa ctttaagaag gagatataca tatggaagat gcctcctctg actcgacggg
       61 tgctgatcct gcccaggaag ctgggacctc taagcctaat gaagagatct caggtccagc
      121 agaaccagct tcacccccag agacaaccac aacagcccag gagacttcgg cggcagcagt
      181 tcaggggaca gccaaggtca cctcaagcag gcaggaacta aaccccctga aatccatagt
      241 ggagaaaagt agcttactaa cagaacaagc ccttgcaaaa gcaggaaaag gaatgcacgg
      301 aggcgtgcca ggtggaaaac aattcatcga aaatggaagt gaatttgcac aaaaattact
      361 gaagaaattc agtctattaa aaccatgggc atgctttgcc aagggtacca atgttttaat
      421 ggcggatggg tctattgaat gtattgaaaa cattgaggtt ggtaataagg tcatgggtaa
      481 agatggcaga cctcgtgagg taattaaatt gcccagagga agagaaacta tgtacagcgt
      541 cgtgcagaaa agtcagcaca gagcccacaa aagtgactca agtcgtgaag tgccagaatt
      601 actcaagttt acgtgtaatg cgacccatga gttggttgtt agaacacctc gtagtgtccg
      661 ccgtttgtct cgtaccatta agggtgtcga atattttgaa gttattactt ttgagatggg
      721 ccaaaagaaa gcccccgacg gtagaattgt tgagcttgtc aaggaagttt caaagagcta
      781 cccaatatct gaggggcctg agagagccaa cgaattagta gaatcctata gaaaggcttc
      841 aaataaagct tattttgagt ggactattg
//
