LOCUS       sequence-72777-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes human-optimized dCas9_VP64 synthetic transcription factor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pCMV_dCas9_VP64
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      283..292
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(321..524)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(350..370)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        525..828
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 agtcatccac cttagccatc tcattactaa agatctcctg caggtagcag atccgattct
       61 ttctgcgggt atatctgcgc cgtgctgttc ttttgagccg cgtggcttcg gccgtctccc
      121 cggagtcgaa caggagggcg ccaatgaggt tcttctttat gctgtggcga tcggtattgc
      181 ccagaacttt gaattttttg ctcggcacct tgtactcgtc cgtaatgacg gcccagccga
      241 cgctgtttgt gccgatggcg agcccaatgg agtacttctt gtccatggtg gcacgcgtgg
      301 atctgacggt tcactaaacc agctctgctt atatagacct cccaccgtac acgcctaccg
      361 cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg
      421 tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca
      481 aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg
      541 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
      601 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
      661 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
      721 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
      781 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
      841 tatgggctat gaactaatga ccccgtaatt gattactatt
//
