LOCUS       sequence-72778-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gRNA expression empty vector (mammalian cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Farzadfard F, Perli SD, Lu TK
  TITLE     Tunable and Multifunctional Eukaryotic Transcription Factors Based 
            on CRISPR/Cas.
  JOURNAL   ACS Synth Biol. 2013 Sep 11.
  PUBMED    23977949
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Sep 11."
COMMENT     Sequence Label: pU6_gRNA_handle_U6t
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(189..293)
                     /gene="bla"
                     /label=AmpR promoter
     promoter        388..636
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     388..408
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     559..578
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        638..713
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     protein_bind    complement(725..749)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(809..832)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
ORIGIN
        1 gttcttcggg gcgaaaactc tcaaggatct taccgctgnt tgagatccag ttcgatgtaa
       61 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      121 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      181 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      241 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      301 ccccgaaaag tgccaccttg tacaaaaaag caggctttaa aggaaccaat tcagtcgact
      361 ggatccggta ccaaggtcgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg
      421 catatacgat acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag
      481 atattagtac aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta
      541 aaattatgtt ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc
      601 ttggctttat atatcttgtg gaaaggacgg agctccggtt ttagagctag aaatagcaag
      661 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
      721 ctagacccag ctttcttgta caaagttggc attagacgtc gaggaattct accgggtagg
      781 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
      841 gcgctacaca agtggcctct ggcctcgcac acattccaca
//
