LOCUS       sequence-72779-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lacritin Protein with Mutation at protein 98
            from Isoleucine to Serine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler 
            MF 4th, Lee JK, Laurie GW
  TITLE     Lacritin rescues stressed epithelia via rapid forkhead box O3 
            (FOXO3)-associated autophagy that restores metabolism.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 
            10.1074/jbc.M112.436584. Epub 2013 May 2.
  PUBMED    23640897
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.436584"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18146-61"
COMMENT     Sequence Label: pTYB1-pLAC-I98S
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             5..27
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 taattttgtt taactttaag aaggagatat acatatggaa gatgcctcct ctgactcgac
       61 gggtgctgat cctgcccagg aagctgggac ctctaagcct aatgaagaga tctcaggtcc
      121 agcagaacca gcttcacccc cagagacaac cacaacagcc caggagactt cggcggcagc
      181 agttcagggg acagccaagg tcacctcaag caggcaggaa ctaaaccccc tgaaatccat
      241 agtggagaaa agtatcttac taacagaaca agcccttgca aaagcaggaa aaggaatgca
      301 cggaggcgtg ccaggtggaa aacaattcag cgaaaatgga agtgaatttg cacaaaaatt
      361 actgaagaaa ttcagtctat taaaaccatg ggcatgcttt gccaagggta ccaatgtttt
      421 aatggcggat gggtctattg aatgtattga aaacattgag gttggtaata aggtcatggg
      481 taaagatggc agacctcgtg aggtaattaa attgcccaga ggaagagaaa ctatgtacag
      541 cgtcgtgcag aaaagtcagc acagagccca caaaagtgac tcaagtcgtg aagtgccaga
      601 attactcaag tttacgtgta atgcgaccca tgagttggtt gttagaacac ctcgtagtgt
      661 ccgccgtttg tctcgtacca ttaagggtgt cgaatatttt gaagttatta cttttgagat
      721 gggccaaaag aaagcccccg acggtagaat tgttgagctt gtcaaggaag tttcaaagag
      781 ctacccaata tctgaggggc ctgagagagc caacgaatta gtagaatcct atagaaa
//
