LOCUS       sequence-72781-e        2990 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Empty destination vector for creating a GreenGate plant 
            transformation plasmid with the plant resistance cassette at the 
            T-DNA right border..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2990)
  AUTHORS   Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner 
            J
  TITLE     GreenGate - A Novel, Versatile, and Efficient Cloning System for 
            Plant Transgenesis.
  JOURNAL   PLoS One. 2013 Dec 20;8(12):e83043. doi: 
            10.1371/journal.pone.0083043.
  PUBMED    24376629
REFERENCE   2  (bases 1 to 2990)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2013-12-20"; volume: "8(12):e83043. doi"; pages: " 
            10.1371/journal.pone.0083043"
COMMENT     Sequence Label: pGGY001
FEATURES             Location/Qualifiers
     source          1..2990
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    6..28
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
     CDS             1589..2248
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1655..1674)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             2267..2572
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     2451..2470
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     primer_bind     complement(2602..2624)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(2818..2837)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    2941..2965
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 gatcttggca ggatatattg tggtgtaacg ttctcgaact aggctcggac gaagtaagcg
       61 gccgcaacct taattaaaca cggcgcgcca acagcgatcg caccaggtac caccttgaga
      121 ccgaattcgc ttactaaaag ccagataaca gtatgcgtat ttgcgcgctg atttttgcgg
      181 tataagaata tatactgata tgtatacccg aagtatgttg gatttgcccc tatatttcca
      241 gacatctgtt atcacttaac ccattacaag cccgctgccg cagatattcc cgtggcgagc
      301 gataacccag cgcactatgc ggatgccatt cgttataatg ctcgaacgcc tctgcaaggt
      361 tctttgctgc cgttaacccg tctggtttgg gcatgatact gatgtagtca cgctttatcg
      421 ttttcacgaa gctctctgct attccgttac tctccggact ccgcaccgcc gtgttcttcg
      481 gttcaagtcc caacatccgg gcgaactggc gtgtttcatt agcccggtag catgaaccat
      541 tatccgtcag ccactccact ggagacgacg gaagatcgtt gccgaagcgg cgttccaccg
      601 ctcccagcat gacgtcctgt actgtttcac tgttgaagcc gccggtagtg accgcccagt
      661 gcagtgcctc acgatcacag cagtccagcg cgaacgtgac acgcagtctc tctccgttat
      721 cacagcagaa ctcgaacccg tcagagcacc atcgctgatt gctttctttc acggccactc
      781 tgcctgtatg tgcccgtttc gatggcggta cagcaggttt tcgctcaagc aacagcgcat
      841 tctggcgcat gatccggtaa acacgtttgg cattgatcgc aggcatacca tcaagttctg
      901 cctgtctgcg aagcagcgcc catacccgac gataaccata cgttggcagc tctccgataa
      961 catggtgtat acggagaagc acatccgtat catcagtgtg acgactgcgg cggccatcca
     1021 tccagtcatc ggttcgtctg agaatgacgt gcaactgcgc acgcgacacc cggagacaac
     1081 ggctgactaa gcttactccc catccccggg caataagggc gcgtgcgcta tccacttttt
     1141 tgcccgtcca tattcaacgg cttctttgag gagttcattt tccatcgttt tcttgccgag
     1201 caggcgctgg agttctttaa tctgcttcat ggcggcagca agttcagagg caggaacaac
     1261 ctgttctccg gcggcgacag cagtaagact tccttcctgg tattgcttac gccagagaaa
     1321 taactggctg gctgctacac catgttgccg ggcaacgagg gagaccgtca tccccggttc
     1381 aaagctctgc tgaacaattg cgatcttttc ctgtgtggta cgccgtctgc gtttctccgg
     1441 ccctaagaca tcaatcatct gttctccaat gactagtcta aaaactagta ttaagactat
     1501 cacttattta agtgatattg gttgtctgga gattcagggg gccagtctat atgtcaaaaa
     1561 gaggtgtgct aagcttagga ggtcagctat ggagaaaaaa atcactggat ataccaccgt
     1621 tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag ttgctcaatg
     1681 tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg taaagaaaaa
     1741 taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga atgctcatcc
     1801 ggagttccgt atggcaatga aagacggtga gctggtgata tgggatagtg ttcacccttg
     1861 ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg aataccacga
     1921 cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg gtgaaaacct
     1981 ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtctcagcca atccctgggt
     2041 gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg cccccgtttt
     2101 caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg cgattcaggt
     2161 tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat tacaacagta
     2221 ctgcgatgag tggcagggcg gggcgtaaga gctcaggagg acagctatgc agtttaaggt
     2281 ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga gtgatattat
     2341 tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc tgtcagataa
     2401 agtctcccgt gaactttacc cagtggtgca tatcggggat gaaagctggc gcatgatgac
     2461 caccgatatg gccagtgtgc cagtctccgt tatcggggaa gaagtggctg atctcagcca
     2521 ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat aaactagtag
     2581 gaggtaatca atgctggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     2641 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     2701 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     2761 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     2821 aatctgctct gatgccgcat gatcatctgg cggacctctt cggaggtccg cttttttttc
     2881 tcgagggtct cagtattcta gaccaagcat gcacagctgg cgttctcagc cagatcccct
     2941 tgacaggata tattggcggg taaactaagt cgctgtatgt gtttgtttga
//
