LOCUS       sequence-72784-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lacritin Protein with Mutation at protein 
            108 from Leucine to Serine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler 
            MF 4th, Lee JK, Laurie GW
  TITLE     Lacritin rescues stressed epithelia via rapid forkhead box O3 
            (FOXO3)-associated autophagy that restores metabolism.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 
            10.1074/jbc.M112.436584. Epub 2013 May 2.
  PUBMED    23640897
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.436584"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18146-61"
COMMENT     Sequence Label: pTYB-pLAC-L108S
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             4..26
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aattttgttt aactttaaga aggagatata catatggaag atgcctcctc tgactcgacg
       61 ggtgctgatc ctgcccagga agctgggacc tctaagccta atgaagagat ctcaggtcca
      121 gcagaaccag cttcaccccc agagacaacc acaacagccc aggagacttc ggcggcagca
      181 gttcagggga cagccaaggt cacctcaagc aggcaggaac taaaccccct gaaatccata
      241 gtggagaaaa gtatcttact aacagaacaa gcccttgcaa aagcaggaaa aggaatgcac
      301 ggaggcgtgc caggtggaaa acaattcatc gaaaatggaa gtgaatttgc acaaaaatca
      361 ctgaagaaat tcagtctatt aaaaccatgg gcatgctttg ccaagggtac caatgtttta
      421 atggcggatg ggtctattga atgtattgaa aacattgagg ttggtaataa ggtcatgggt
      481 aaagatggca gacctcgtga ggtaattaaa ttgcccagag gaagagaaac tatgtacagc
      541 gtcgtgcaga aaagtcagca cagagcccac aaaagtgact caagtcgtga agtgccagaa
      601 ttactcaagt ttacgtgtaa tgcgacccat gagttggttg ttagaacacc tcgtagtgtc
      661 cgccgtttgt ctcgtaccat taagggtgtc gaatattttg aagttattac ttttgagatg
      721 ggccaaaaga aagcccccga cggtagaatt gttgagcttg tcaaggaagt ttcaaagagc
      781 tacccaatat ctgaggggcc tgagagagcc aacgaattag tagaatccta tagaaaggct
      841 tcaaataaag cttattttga gtggactatt g
//
