LOCUS       sequence-72786-e         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lacritin Protein with Mutation at protein 
            112 from Phenylalanine to Serine .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler 
            MF 4th, Lee JK, Laurie GW
  TITLE     Lacritin rescues stressed epithelia via rapid forkhead box O3 
            (FOXO3)-associated autophagy that restores metabolism.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 
            10.1074/jbc.M112.436584. Epub 2013 May 2.
  PUBMED    23640897
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.436584"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18146-61"
COMMENT     Sequence Label: pTYB-pLAC-F112S
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             6..28
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ataattttgt ttaactttaa gaaggagata tacatatgga agatgcctcc tctgactcga
       61 cgggtgctga tcctgcccag gaagctggga cctctaagcc taatgaagag atctcaggtc
      121 cagcagaacc agcttcaccc ccagagacaa ccacaacagc ccaggagact tcggcggcag
      181 cagttcaggg gacagccaag gtcacctcaa gcaggcagga actaaacccc ctgaaatcca
      241 tagtggagaa aagtatctta ctaacagaac aagcccttgc aaaagcagga aaaggaatgc
      301 acggaggcgt gccaggtgga aaacaattca tcgaaaatgg aagtgaattt gcacaaaaat
      361 tactgaagaa atccagtcta ttaaaaccat gggcatgctt tgccaagggt accaatgttt
      421 taatggcgga tgggtctatt gaatgtattg aaaacattga ggttggtaat aaggtcatgg
      481 gtaaagatgg cagacctcgt gaggtaatta aattgcccag aggaagagaa actatgtaca
      541 gcgtcgtgca gaaaagtcag cacagagccc acaaaagtga ctcaagtcgt gaagtgccag
      601 aattactcaa gtttacgtgt aatgcgaccc atgagttggt tgttagaaca cctcgtagtg
      661 tccgccgttt gtctcgtacc attaagggtg tcgaatattt tgaagttatt acttttgaga
      721 tgggccaaaa gaaagccccc gacggtagaa ttgttgagct tgtcaaggaa gtttcaaaga
      781 gctacccaat atctgagggg cctgagagag ccaacgaatt agtagaatcc tatagaaagg
      841 cttcaaataa agcttatttt gagtggacta ttgaggccag agatctttct ctgttgggtt
      901 cccatgttcg taaagctacc taca
//
