LOCUS       sequence-72787-e         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lacritin Proteins with Mutation at protein 
            68 and 73 from Isoleucine to Serine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler 
            MF 4th, Lee JK, Laurie GW
  TITLE     Lacritin rescues stressed epithelia via rapid forkhead box O3 
            (FOXO3)-associated autophagy that restores metabolism.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 
            10.1074/jbc.M112.436584. Epub 2013 May 2.
  PUBMED    23640897
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.436584"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18146-61"
COMMENT     Sequence Label: pTYB-pLAC-I68S/I73S
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatggaagat gcctcctctg
       61 actcgacggg tgctgatcct gcccaggaag ctgggacctc taagcctaat gaagagatct
      121 caggtccagc agaaccagct tcacccccag agacaaccac aacagcccag gagacttcgg
      181 cggcagcagt tcaggggaca gccaaggtca cctcaagcag gcaggaacta aaccccctga
      241 aatccagcgt ggagaaaagt agcttactaa cagaacaagc ccttgcaaaa gcaggaaaag
      301 gaatgcacgg aggcgtgcca ggtggaaaac aattcatcga aaatggaagt gaatttgcac
      361 aaaaattact gaagaaattc agtctattaa aaccatgggc atgctttgcc aagggtacca
      421 atgttttaat ggcggatggg tctattgaat gtattgaaaa cattgaggtt ggtaataagg
      481 tcatgggtaa agatggcaga cctcgtgagg taattaaatt gcccagagga agagaaacta
      541 tgtacagcgt cgtgcagaaa agtcagcaca gagcccacaa aagtgactca agtcgtgaag
      601 tgccagaatt actcaagttt acgtgtaatg cgacccatga gttggttgtt agaacacctc
      661 gtagtgtccg ccgtttgtct cgtaccatta agggtgtcga atattttgaa gttattactt
      721 ttgagatggg ccaaaagaaa gcccccgacg gtagaattgt tgagcttgtc aaggaagttt
      781 caaagagcta cccaatatct gaggggcctg agagagccaa cgaattagta gaatcctata
      841 gaaaggcttc aaataaagct tattttgagt ggactattga ggccagagat ctttctctgt
      901 tgggttccca tgttcgtaaa gctacctacc agacttac
//
