LOCUS       sequence-72792-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of Lacritin Protein with Mutation at proteins 
            108 and 109 Leucine to Serine and 112 from phenylalanine to serine.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Wang N, Zimmerman K, Raab RW, McKown RL, Hutnik CM, Talla V, Tyler 
            MF 4th, Lee JK, Laurie GW
  TITLE     Lacritin rescues stressed epithelia via rapid forkhead box O3 
            (FOXO3)-associated autophagy that restores metabolism.
  JOURNAL   J Biol Chem. 2013 Jun 21;288(25):18146-61. doi: 
            10.1074/jbc.M112.436584. Epub 2013 May 2.
  PUBMED    23640897
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M112.436584"; journalName: "J Biol Chem"; date: 
            "2013-06-21- 21"; volume: "288"; issue: "25"; pages: "18146-61"
COMMENT     Sequence Label: pTYB-pLAC-L108S/L109S/F112S
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             7..29
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 aataattttg tttaacttta agaaggagat atacatatgg aagatgcctc ctctgactcg
       61 acgggtgctg atcctgccca ggaagctggg acctctaagc ctaatgaaga gatctcaggt
      121 ccagcagaac cagcttcacc cccagagaca accacaacag cccaggagac ttcggcggca
      181 gcagttcagg ggacagccaa ggtcacctca agcaggcagg aactaaaccc cctgaaatcc
      241 atagtggaga aaagtatctt actaacagaa caagcccttg caaaagcagg aaaaggaatg
      301 cacggaggcg tgccaggtgg aaaacaattc atcgaaaatg gaagtgaatt tgcacaaaaa
      361 tcatcgaaga aatccagtct attaaaacca tgggcatgct ttgccaaggg taccaatgtt
      421 ttaatggcgg atgggtctat tgaatgtatt gaaaacattg aggttggtaa taaggtcatg
      481 ggtaaagatg gcagacctcg tgaggtaatt aaattgccca gaggaagaga aactatgtac
      541 agcgtcgtgc agaaaagtca gcacagagcc cacaaaagtg actcaagtcg tgaagtgcca
      601 gaattactca agtttacgtg taatgcgacc catgagttgg ttgttagaac acctcgtagt
      661 gtccgccgtt tgtctcgtac cattaagggt gtcgaatatt ttgaagttat tacttttgag
      721 atgggccaaa agaaagcccc cgacggtaga attgttgagc ttgtcaagga agtttcaaag
      781 agctacccaa tatctgaggg gcctgagaga gccaacgaat tagtagaatc ctatagaaag
      841 gcttcaaata aagcttattt tgagtggact attgaggcca gagatctttc tctgttgggt
      901 tcccatgttc gtaaagctac ctaccagact tacgctcca
//
